TY - JOUR
T1 - Role of modulator recognition factor 2 in adipogenesis and leptin expression in 3T3-L1 cells
AU - Dong, Jie
AU - Ishimori, Naoki
AU - Paigen, Beverly
AU - Tsutsui, Hiroyuki
AU - Fujii, Satoshi
N1 - Funding Information:
This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sport, Science and Technology of Japan. D.G. is a fellow of the JSPS during the tenure of this work. The technical assistance of Ms. Miwako Fujii is greatly appreciated.
PY - 2008/2/8
Y1 - 2008/2/8
N2 - The complex network of adipogenic transcription factors regulates adipocyte differentiation, obesity, and insulin resistance. Modulator recognition factor (Mrf) 2 knockout mice exhibit defects in fat accumulation and are protected from diet-induced obesity, suggesting that Mrf2 deficiency affects adipogenesis. Here, we report that the gene expressions of the 2 isoforms of the transcription factors Mrf2, Mrf2α, and Mrf2β, were induced upon adipogenesis in 3T3-L1 cells. Mrf2 mRNA expression was sensitive to stimulation by insulin, dexamethasone, and TNF-α in 3T3-L1 preadipocytes and differentiated adipocytes. Down-regulation of Mrf2α and Mrf2β gene expressions induced by small interfering RNAs increased the mRNA expression of leptin. These results indicate that Mrf2 can be a potential regulator of adipocyte differentiation and a potential repressor of leptin.
AB - The complex network of adipogenic transcription factors regulates adipocyte differentiation, obesity, and insulin resistance. Modulator recognition factor (Mrf) 2 knockout mice exhibit defects in fat accumulation and are protected from diet-induced obesity, suggesting that Mrf2 deficiency affects adipogenesis. Here, we report that the gene expressions of the 2 isoforms of the transcription factors Mrf2, Mrf2α, and Mrf2β, were induced upon adipogenesis in 3T3-L1 cells. Mrf2 mRNA expression was sensitive to stimulation by insulin, dexamethasone, and TNF-α in 3T3-L1 preadipocytes and differentiated adipocytes. Down-regulation of Mrf2α and Mrf2β gene expressions induced by small interfering RNAs increased the mRNA expression of leptin. These results indicate that Mrf2 can be a potential regulator of adipocyte differentiation and a potential repressor of leptin.
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U2 - 10.1016/j.bbrc.2007.12.002
DO - 10.1016/j.bbrc.2007.12.002
M3 - Article
C2 - 18070594
AN - SCOPUS:37449012218
SN - 0006-291X
VL - 366
SP - 551
EP - 555
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
