Role of poly(ADP-ribose) polymerase in cisplatin-induced injury in LLC-PK1 cells

Yuki Shino, Yoshinori Itoh, Toshio Kubota, Takahisa Yano, Toshiaki Sendo, Ryozo Oishi

Research output: Contribution to journalArticle

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Abstract

Acute renal failure is a dose-limiting factor during cisplatin chemotherapy. We have previously shown in rats that the hydroxyl radical scavenger edaravone reverses cisplatin-induced in vivo renal damage. In the present study, the role of poly(ADP-ribose) polymerase (PARP) in cisplatin nephrotoxicity was investigated in porcine tubular cells LLC-PK1. Cell injury was elicited by transient exposure to 500 μM cisplatin for 1 h or continuous exposure to 30 μM cisplatin for 24 h. Various hydroxyl radical scavengers reversed cell damage in a transient but not permanent model. The cell injury seemed to be necrosis and apoptosis in transient and permanent models, respectively, as assessed by TUNEL method and Annexin V stain. PARP inhibitors such as 3-aminobenzamide and benzamide inhibited cell damage in transient but not permanent model. PARP-dependent cell injury was also observed after transient exposure to hydroxyl radical-generating solution. We demonstrated for the first time the activation of PARP in renal tubular cells by transient cisplatin exposure, as determined by immunofluorescent stain with anti-poly(ADP-ribose) antibody. Moreover, ATP was depleted by transient exposure to cisplatin or hydroxyl radical, both of which were reversed by PARP inhibitors. These findings suggest that hydroxyl radical generation followed by PARP activation contributes to the necrotic cell injury caused by a transient exposure to cisplatin.

Original languageEnglish
Pages (from-to)966-977
Number of pages12
JournalFree Radical Biology and Medicine
Volume35
Issue number8
DOIs
Publication statusPublished - Oct 15 2003

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LLC-PK1 Cells
Poly(ADP-ribose) Polymerases
Cisplatin
Hydroxyl Radical
Wounds and Injuries
Coloring Agents
Chemical activation
Cells
Poly Adenosine Diphosphate Ribose
Kidney
Chemotherapy
Annexin A5
In Situ Nick-End Labeling
Acute Kidney Injury
Rats
Necrosis
Swine
Adenosine Triphosphate
Apoptosis
Drug Therapy

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

Cite this

Role of poly(ADP-ribose) polymerase in cisplatin-induced injury in LLC-PK1 cells. / Shino, Yuki; Itoh, Yoshinori; Kubota, Toshio; Yano, Takahisa; Sendo, Toshiaki; Oishi, Ryozo.

In: Free Radical Biology and Medicine, Vol. 35, No. 8, 15.10.2003, p. 966-977.

Research output: Contribution to journalArticle

Shino, Yuki ; Itoh, Yoshinori ; Kubota, Toshio ; Yano, Takahisa ; Sendo, Toshiaki ; Oishi, Ryozo. / Role of poly(ADP-ribose) polymerase in cisplatin-induced injury in LLC-PK1 cells. In: Free Radical Biology and Medicine. 2003 ; Vol. 35, No. 8. pp. 966-977.
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