Role of PRIP-1, a novel Ins(1,4,5)P3 binding protein, in Ins(1,4,5)P3-mediated Ca2+ signaling

Kae Harada, Hiroshi Takeuchi, Masahiro Oike, Miho Matsuda, Takashi Kanematsu, Hitoshi Yagisawa, Kei Ichi I. Nakayama, Katsumasa Maeda, Christophe Erneux, Masato Hirata

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Abstract

PRIP-1 was isolated as a novel inositol 1,4,5-trisphosphate [ins(1,4,5)P3] binding protein with a domain organization similar to phospholipase C-δ1 (PLC-δ1) but lacking the enzymatic activity. Further studies revealed that the pleckstrin homology (PH) domain of PRIP-1 is the region responsible for binding Ins(1,4,5)P3. In this study we aimed to clarify the role of PRIP-1 at the physiological concentration in Ins(1,4,5)P3-mediated Ca2+ signaling, as we had previously used COS-1 cells overexpressing PRIP-1 (Takeuchi et al., 2000, Biochem J 349:357-368). For this purpose we employed PRIP-1 knock out (PRIP-1 -/-) mice generated previously (Kanematsu et al., 2002, EMBO J 21:1004-1011). The increase in free Ca2+ concentration in response to purinergic receptor stimulation was lower in primary cultured cortical neurons prepared from PRIP-1-/- mice than in those from wild type mice. The relative amounts of [3H]Ins(1,4,5)P3 measured in neurons labeled with [3H]inositol was also lower in cells from PRIP-1 -/- mice. In contrast, PLC activities in brain cortex samples from PRIP-1-/- mice were not different from those in the wild type mice, indicating that the hydrolysis of Ins(1,4,S)P3 is enhanced in cells from PRIP-1-/- mice. In vitro analyses revealed that type1 inositol polyphosphate 5-phosphatase physically interacted with a PH domain of PRIP-1 (PRIP-1PH) and its enzyme activity was inhibited by PRIP-1 PH. However, physical interaction with these two proteins did not appear to be the reason for the inhibition of enzyme activity, indicating that binding of Ins(1,4,5)P 3 to the PH domain prevented its hydrolyzation. Together, these results indicate that PRIP-1 plays an important role in regulating the Ins(1,4,5)P3-mediated Ca2+ signaling by modulating type1 inositol polyphosphate 5-phosphatase activity through binding to Ins(1,4,5)P3.

Original languageEnglish
Pages (from-to)422-433
Number of pages12
JournalJournal of cellular physiology
Volume202
Issue number2
DOIs
Publication statusPublished - Feb 1 2005

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Carrier Proteins
Inositol
Polyphosphates
Enzyme activity
Phosphoric Monoester Hydrolases
Neurons
Enzyme inhibition
Purinergic Receptors
Inositol 1,4,5-Trisphosphate
Type C Phospholipases
Programmable logic controllers
Hydrolysis
Brain
COS Cells
Enzymes
platelet protein P47
Proteins
Pleckstrin Homology Domains
Inositol Polyphosphate 5-Phosphatases

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Role of PRIP-1, a novel Ins(1,4,5)P3 binding protein, in Ins(1,4,5)P3-mediated Ca2+ signaling. / Harada, Kae; Takeuchi, Hiroshi; Oike, Masahiro; Matsuda, Miho; Kanematsu, Takashi; Yagisawa, Hitoshi; Nakayama, Kei Ichi I.; Maeda, Katsumasa; Erneux, Christophe; Hirata, Masato.

In: Journal of cellular physiology, Vol. 202, No. 2, 01.02.2005, p. 422-433.

Research output: Contribution to journalArticle

Harada, Kae ; Takeuchi, Hiroshi ; Oike, Masahiro ; Matsuda, Miho ; Kanematsu, Takashi ; Yagisawa, Hitoshi ; Nakayama, Kei Ichi I. ; Maeda, Katsumasa ; Erneux, Christophe ; Hirata, Masato. / Role of PRIP-1, a novel Ins(1,4,5)P3 binding protein, in Ins(1,4,5)P3-mediated Ca2+ signaling. In: Journal of cellular physiology. 2005 ; Vol. 202, No. 2. pp. 422-433.
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abstract = "PRIP-1 was isolated as a novel inositol 1,4,5-trisphosphate [ins(1,4,5)P3] binding protein with a domain organization similar to phospholipase C-δ1 (PLC-δ1) but lacking the enzymatic activity. Further studies revealed that the pleckstrin homology (PH) domain of PRIP-1 is the region responsible for binding Ins(1,4,5)P3. In this study we aimed to clarify the role of PRIP-1 at the physiological concentration in Ins(1,4,5)P3-mediated Ca2+ signaling, as we had previously used COS-1 cells overexpressing PRIP-1 (Takeuchi et al., 2000, Biochem J 349:357-368). For this purpose we employed PRIP-1 knock out (PRIP-1 -/-) mice generated previously (Kanematsu et al., 2002, EMBO J 21:1004-1011). The increase in free Ca2+ concentration in response to purinergic receptor stimulation was lower in primary cultured cortical neurons prepared from PRIP-1-/- mice than in those from wild type mice. The relative amounts of [3H]Ins(1,4,5)P3 measured in neurons labeled with [3H]inositol was also lower in cells from PRIP-1 -/- mice. In contrast, PLC activities in brain cortex samples from PRIP-1-/- mice were not different from those in the wild type mice, indicating that the hydrolysis of Ins(1,4,S)P3 is enhanced in cells from PRIP-1-/- mice. In vitro analyses revealed that type1 inositol polyphosphate 5-phosphatase physically interacted with a PH domain of PRIP-1 (PRIP-1PH) and its enzyme activity was inhibited by PRIP-1 PH. However, physical interaction with these two proteins did not appear to be the reason for the inhibition of enzyme activity, indicating that binding of Ins(1,4,5)P 3 to the PH domain prevented its hydrolyzation. Together, these results indicate that PRIP-1 plays an important role in regulating the Ins(1,4,5)P3-mediated Ca2+ signaling by modulating type1 inositol polyphosphate 5-phosphatase activity through binding to Ins(1,4,5)P3.",
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AU - Oike, Masahiro

AU - Matsuda, Miho

AU - Kanematsu, Takashi

AU - Yagisawa, Hitoshi

AU - Nakayama, Kei Ichi I.

AU - Maeda, Katsumasa

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