Role of reactive oxygen species produced by NADPH oxidase in gibberellin biosynthesis during barley seed germination

Kyohei Kai, Shinsuke Kasa, Masatsugu Sakamoto, Nozomi Aoki, Gaku Watabe, Takashi Yuasa, Mari Iwaya-Inoue, Yushi Ishibashi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

NADPH oxidase catalyzes the production of the superoxide anion (O2 ), a reactive oxygen species (ROS), and regulates the germination of barley (Hordeum vulgare L.). Diphenyleneiodonium (DPI) chloride, an NADPH oxidase inhibitor, delayed barley germination, and exogenous H2O2 (an ROS) partially rescued it. Six enzymes, ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurene oxidase (KO), ent-kaurenoic acid oxidase (KAO), GA20-oxidase (GA20ox) and GA3-oxidase (GA3ox), catalyze the transformation of trans-geranylgeranyl diphosphate to active gibberellin, which promotes germination. Exogenous H2O2 promoted the expressions of HvKAO1 and HvGA3ox1 in barley embryos. These results suggest that ROS produced by NADPH oxidase are involved in gibberellin biosynthesis through the regulation of HvKAO1 and HvGA3ox1.

Original languageEnglish
Article numbere1180492
JournalPlant Signaling and Behavior
Volume11
Issue number5
DOIs
Publication statusPublished - May 3 2016

All Science Journal Classification (ASJC) codes

  • Plant Science

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