Role of serotype-specific polysaccharide in the resistance of Streptococcus mutans to phagocytosis by human polymorphonuclear leukocytes

Hiromasa Tsuda, Yoshihisa Yamashita, Kuniaki Toyoshima, Noboru Yamaguchi, Takahiko Oho, Yoshio Nakano, Kengo Nagata, Toshihiko Koga

Research output: Contribution to journalArticle

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Abstract

To clarify the role of cell surface components of Streptococcus mutans in resistance to phagocytosis by human polymorphonuclear leukocytes (PMNs), several isogenic mutants of S. mutans defective in cell surface components were studied with a luminol-enhanced chemiluminescence (CL) assay, a killing assay, and a transmission electron microscope. The CL responses of human PMNs to mutant Xc11 defective in a major cell surface antigen, PAc, and mutant Xc16 defective in two surface glucosyltransferases (GTF-I and GTF-SI) were the same as the response to the wild-type strain, Xc. In contrast, mutant Xc24R, which was defective in serotype c-specific polysaccharide, induced a markedly higher CL response than the other strains. The killing assay showed that human PMNs killed more Xc24R than the parent strain and the other mutants. The transmission electron microscopic observation indicated that Xc24R cells were more internalized by human PMNs than the parental strain Xc. These results may be reflected by the fact that strain Xc24R was more phagocytosed than strain Xc. The CL response of human PMNs to a mutant defective in polysaccharide serotype e or f was similar Z to the response to Xc24R. Furthermore, mutants defective in serotype-specific polysaccharide were markedly more hydrophobic than the wild-type strains and the other mutants, suggesting that the hydrophilic nature of polysaccharides may protect the bacterium from phagocytosis. We conclude that the serotype- specific polysaccharide, but not the cell surface proteins on the cell surface of S. mutans, may play an important role in the resistance to phagocytosis.

Original languageEnglish
Pages (from-to)644-650
Number of pages7
JournalInfection and Immunity
Volume68
Issue number2
DOIs
Publication statusPublished - Feb 1 2000

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Streptococcus mutans
Phagocytosis
Polysaccharides
Luminescence
Neutrophils
Cellular Structures
Electrons
Luminol
Surface Antigens
Membrane Proteins
Serogroup
Bacteria

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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Role of serotype-specific polysaccharide in the resistance of Streptococcus mutans to phagocytosis by human polymorphonuclear leukocytes. / Tsuda, Hiromasa; Yamashita, Yoshihisa; Toyoshima, Kuniaki; Yamaguchi, Noboru; Oho, Takahiko; Nakano, Yoshio; Nagata, Kengo; Koga, Toshihiko.

In: Infection and Immunity, Vol. 68, No. 2, 01.02.2000, p. 644-650.

Research output: Contribution to journalArticle

Tsuda, Hiromasa ; Yamashita, Yoshihisa ; Toyoshima, Kuniaki ; Yamaguchi, Noboru ; Oho, Takahiko ; Nakano, Yoshio ; Nagata, Kengo ; Koga, Toshihiko. / Role of serotype-specific polysaccharide in the resistance of Streptococcus mutans to phagocytosis by human polymorphonuclear leukocytes. In: Infection and Immunity. 2000 ; Vol. 68, No. 2. pp. 644-650.
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