Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans

Y. Murakami, Yoshihisa Yamashita, Y. Nakano, H. O. Ito, H. Yu, T. Koga

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Abstract

To make clear the role of the C terminus of a surface protein antigen (PAc) of Streptococcus mutans, stepwise truncations beginning at the C terminus of PAc were performed by utilizing site-directed mutagenesis. A remarkable increase in the amount of cell-free PAc was observed upon deletion of four or more amino acid residues at the C terminus. On the other hand, the amount of cell surface PAc gradually decreased when increasing numbers (four or more) of amino acid residues were deleted at the C terminus, and deletion of six amino acids involving both the total charged tail and Leu, an amino acid residue immediately upstream of the charged tail, resulted in a drastic reduction in the amount of cell surface PAc. These results indicate that the cytoplasmic charged tail and Leu residue are required for cell surface localization of PAc in S. mutans.

Original languageEnglish
Pages (from-to)1531-1535
Number of pages5
JournalInfection and Immunity
Volume65
Issue number4
Publication statusPublished - Apr 15 1997

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All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Murakami, Y., Yamashita, Y., Nakano, Y., Ito, H. O., Yu, H., & Koga, T. (1997). Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans. Infection and Immunity, 65(4), 1531-1535.