Role of the Esicherichia coli RecQ DNA helicase in SOS signaling and genome stabiliziation at stalled replication forks

Takashi Hishida, Yong Woon Han, Tatsuya Shibata, Yoshino Kubota, Yoshizumi Ishino, Hiroshi Iwasaki, Hideo Shinagawa

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

The RecQ protein family is a highly conserved group of DNA helicases that play roles in maintaining genomic stability. In this study, we present biochemical and genetic evidence that Escherichia coli RecQ processes stalled replication forks and participates in SOS signaling. Cells that carry dnaE486, a mutation in the DNA polymerase III α-catalytic subunit, induce an RecA-dependent SOS response and become highly filamented at the semirestrictive temperature (38°C). An recQ mutation suppresses the induction of SOS response and the filamentation in the dnaE486 mutant at 38°C, causing appearance of a high proportion of anucleate cells. In vitro, RecQ binds and unwinds forked DNA substrates with a gap on the leading strand more efficiently than those with a gap on the lagging strand or Holliday junction DNA. RecQ unwinds the template duplex ahead of the fork, and then the lagging strand is unwound. Consequently, this process generates a single-stranded DNA (ssDNA) gap on the lagging strand adjacent to a replication fork. These results suggest that RecQ functions to generate an initiating signal that can recruit RecA for SOS induction and recombination at stalled replication forks, which are required for the cell cycle checkpoint and resumption of DNA replication.

Original languageEnglish
Pages (from-to)1886-1897
Number of pages12
JournalGenes and Development
Volume18
Issue number15
DOIs
Publication statusPublished - Aug 1 2004

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RecQ Helicases
DNA Helicases
Genome
Cruciform DNA
DNA Polymerase III
Mutation
Genomic Instability
Single-Stranded DNA
Cell Cycle Checkpoints
DNA Replication
Genetic Recombination
Molecular Biology
Catalytic Domain
Escherichia coli
Temperature
DNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Role of the Esicherichia coli RecQ DNA helicase in SOS signaling and genome stabiliziation at stalled replication forks. / Hishida, Takashi; Han, Yong Woon; Shibata, Tatsuya; Kubota, Yoshino; Ishino, Yoshizumi; Iwasaki, Hiroshi; Shinagawa, Hideo.

In: Genes and Development, Vol. 18, No. 15, 01.08.2004, p. 1886-1897.

Research output: Contribution to journalArticle

Hishida, Takashi ; Han, Yong Woon ; Shibata, Tatsuya ; Kubota, Yoshino ; Ishino, Yoshizumi ; Iwasaki, Hiroshi ; Shinagawa, Hideo. / Role of the Esicherichia coli RecQ DNA helicase in SOS signaling and genome stabiliziation at stalled replication forks. In: Genes and Development. 2004 ; Vol. 18, No. 15. pp. 1886-1897.
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