Role of the liver in determining alloimmune response in vitro following donor-specific spleen cell injection

Masaki Okuyama, Hiroaki Nagano, Shogo Kobayashi, Li He, Hirofumi Ota, Junzo Shimizu, Yutaka Takeda, Shigeru Masrubashi, Hidetoshi Eguchi, Masahiro Tanemuea, Keizo Dono, Masato Sakon, Koji Umeshita, Mitsukazu Gotoh, Morito Monden

Research output: Contribution to journalArticle

Abstract

The aim of our study was to investigate the allogeneic influence inside and outside the liver in vitro following donor-specific cell injection (DSI). DA rats (RT1a) were used as donors and WS rats (RT1k) as recipients. WS were sensitized with DA spleen cells, followed 24. h later by total hepatectomy. The liver was transplanted into another WS (sensitized liver-grafted; SL-Grafted). The hepatectomized WS underwent liver transplantation from a naive WS (sensitized liver-removed; SL-Removed). Alloantigens accumulated in the liver in SL-Grafted and in the extrahepatic tissue/organ(s) in SL-Removed. DA hearts were transplanted 10. days after antigen administration. To analyze the immune responses, we measured Th1/Th2 cytokine profiles, and perforin mRNA in various organs, allogeneic mixed lymphocyte reaction (MLR), and donor-specific immunoglobulin. Th1 cytokine levels in the liver of SL-Grafted and in spleen of SL-Removed were highly and rapidly upregulated but decreased thereafter. IFN-γ and perforin mRNAs were significantly higher in SL-Grafted and lower in SL-Removed. MLR was significantly higher in SL-Grafted than SL-Removed and controls. There was no significant difference in the donor-specific immunoglobulin level. Our findings suggest that liver and other organs may behave differently to alloantigen, suggesting the importance of an early Th1 reaction in the liver and spleen.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalTransplant Immunology
Volume22
Issue number3-4
DOIs
Publication statusPublished - Feb 2010

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Transplantation

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    Okuyama, M., Nagano, H., Kobayashi, S., He, L., Ota, H., Shimizu, J., Takeda, Y., Masrubashi, S., Eguchi, H., Tanemuea, M., Dono, K., Sakon, M., Umeshita, K., Gotoh, M., & Monden, M. (2010). Role of the liver in determining alloimmune response in vitro following donor-specific spleen cell injection. Transplant Immunology, 22(3-4), 150-156. https://doi.org/10.1016/j.trim.2009.10.003