Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells

Karine Tréguer, Eva Marie Heinrich, Kisho Ohtani, Angelika Bonauer, Stefanie Dimmeler

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43 Citations (Scopus)

Abstract

MicroRNAs (miRs) are small non-coding RNAs that recently emerged as potent regulators of gene expression. The members of the miR-17-92 cluster have been shown to control endothelial cell functions and neovascularization; however, the regulation and function of the cluster in endothelial cell lineage commitment has not been explored. This project aimed to test the role of the miR-17-92 cluster during endothelial differentiation. We demonstrate that miR-17, miR-18, miR-19 and miR-20 are increased upon the induction of endothelial cell differentiation of murine embryonic stem cells or induced pluripotent stem cells. In contrast, miR-92a and the primary miR-17-92 transcript were downregulated. The inhibition of each individual miR of the cluster by cholesterol-modified antagomirs did not affect endothelial marker gene expression. Moreover, the combination of all antagomirs had no effect. These findings illustrate that although the miR-17-92 cluster regulates vascular integrity and angiogenesis, none of the members has a significant impact on the endothelial differentiation of pluripotent stem cells.

Original languageEnglish
Pages (from-to)447-460
Number of pages14
JournalJournal of Vascular Research
Volume49
Issue number5
DOIs
Publication statusPublished - Aug 1 2012

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

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    Tréguer, K., Heinrich, E. M., Ohtani, K., Bonauer, A., & Dimmeler, S. (2012). Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells. Journal of Vascular Research, 49(5), 447-460. https://doi.org/10.1159/000339429