Abstract
MicroRNAs (miRs) are small non-coding RNAs that recently emerged as potent regulators of gene expression. The members of the miR-17-92 cluster have been shown to control endothelial cell functions and neovascularization; however, the regulation and function of the cluster in endothelial cell lineage commitment has not been explored. This project aimed to test the role of the miR-17-92 cluster during endothelial differentiation. We demonstrate that miR-17, miR-18, miR-19 and miR-20 are increased upon the induction of endothelial cell differentiation of murine embryonic stem cells or induced pluripotent stem cells. In contrast, miR-92a and the primary miR-17-92 transcript were downregulated. The inhibition of each individual miR of the cluster by cholesterol-modified antagomirs did not affect endothelial marker gene expression. Moreover, the combination of all antagomirs had no effect. These findings illustrate that although the miR-17-92 cluster regulates vascular integrity and angiogenesis, none of the members has a significant impact on the endothelial differentiation of pluripotent stem cells.
| Original language | English |
|---|---|
| Pages (from-to) | 447-460 |
| Number of pages | 14 |
| Journal | Journal of Vascular Research |
| Volume | 49 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Aug 1 2012 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Cardiology and Cardiovascular Medicine
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Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells. / Tréguer, Karine; Heinrich, Eva Marie; Ohtani, Kisho; Bonauer, Angelika; Dimmeler, Stefanie.
In: Journal of Vascular Research, Vol. 49, No. 5, 01.08.2012, p. 447-460.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells
AU - Tréguer, Karine
AU - Heinrich, Eva Marie
AU - Ohtani, Kisho
AU - Bonauer, Angelika
AU - Dimmeler, Stefanie
PY - 2012/8/1
Y1 - 2012/8/1
N2 - MicroRNAs (miRs) are small non-coding RNAs that recently emerged as potent regulators of gene expression. The members of the miR-17-92 cluster have been shown to control endothelial cell functions and neovascularization; however, the regulation and function of the cluster in endothelial cell lineage commitment has not been explored. This project aimed to test the role of the miR-17-92 cluster during endothelial differentiation. We demonstrate that miR-17, miR-18, miR-19 and miR-20 are increased upon the induction of endothelial cell differentiation of murine embryonic stem cells or induced pluripotent stem cells. In contrast, miR-92a and the primary miR-17-92 transcript were downregulated. The inhibition of each individual miR of the cluster by cholesterol-modified antagomirs did not affect endothelial marker gene expression. Moreover, the combination of all antagomirs had no effect. These findings illustrate that although the miR-17-92 cluster regulates vascular integrity and angiogenesis, none of the members has a significant impact on the endothelial differentiation of pluripotent stem cells.
AB - MicroRNAs (miRs) are small non-coding RNAs that recently emerged as potent regulators of gene expression. The members of the miR-17-92 cluster have been shown to control endothelial cell functions and neovascularization; however, the regulation and function of the cluster in endothelial cell lineage commitment has not been explored. This project aimed to test the role of the miR-17-92 cluster during endothelial differentiation. We demonstrate that miR-17, miR-18, miR-19 and miR-20 are increased upon the induction of endothelial cell differentiation of murine embryonic stem cells or induced pluripotent stem cells. In contrast, miR-92a and the primary miR-17-92 transcript were downregulated. The inhibition of each individual miR of the cluster by cholesterol-modified antagomirs did not affect endothelial marker gene expression. Moreover, the combination of all antagomirs had no effect. These findings illustrate that although the miR-17-92 cluster regulates vascular integrity and angiogenesis, none of the members has a significant impact on the endothelial differentiation of pluripotent stem cells.
UR - http://www.scopus.com/inward/record.url?scp=84863771130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863771130&partnerID=8YFLogxK
U2 - 10.1159/000339429
DO - 10.1159/000339429
M3 - Article
C2 - 22797777
AN - SCOPUS:84863771130
VL - 49
SP - 447
EP - 460
JO - Journal of Vascular Research
JF - Journal of Vascular Research
SN - 1018-1172
IS - 5
ER -