Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells

Karine Tréguer, Eva Marie Heinrich, Kisho Ohtani, Angelika Bonauer, Stefanie Dimmeler

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

MicroRNAs (miRs) are small non-coding RNAs that recently emerged as potent regulators of gene expression. The members of the miR-17-92 cluster have been shown to control endothelial cell functions and neovascularization; however, the regulation and function of the cluster in endothelial cell lineage commitment has not been explored. This project aimed to test the role of the miR-17-92 cluster during endothelial differentiation. We demonstrate that miR-17, miR-18, miR-19 and miR-20 are increased upon the induction of endothelial cell differentiation of murine embryonic stem cells or induced pluripotent stem cells. In contrast, miR-92a and the primary miR-17-92 transcript were downregulated. The inhibition of each individual miR of the cluster by cholesterol-modified antagomirs did not affect endothelial marker gene expression. Moreover, the combination of all antagomirs had no effect. These findings illustrate that although the miR-17-92 cluster regulates vascular integrity and angiogenesis, none of the members has a significant impact on the endothelial differentiation of pluripotent stem cells.

Original languageEnglish
Pages (from-to)447-460
Number of pages14
JournalJournal of Vascular Research
Volume49
Issue number5
DOIs
Publication statusPublished - Aug 1 2012

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MicroRNAs
Stem Cells
Endothelial Cells
Gene Expression
Small Untranslated RNA
Induced Pluripotent Stem Cells
Pluripotent Stem Cells
Cell Lineage
Regulator Genes
Embryonic Stem Cells
Blood Vessels
Cell Differentiation
Down-Regulation
Cholesterol
Antagomirs

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Tréguer, K., Heinrich, E. M., Ohtani, K., Bonauer, A., & Dimmeler, S. (2012). Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells. Journal of Vascular Research, 49(5), 447-460. https://doi.org/10.1159/000339429

Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells. / Tréguer, Karine; Heinrich, Eva Marie; Ohtani, Kisho; Bonauer, Angelika; Dimmeler, Stefanie.

In: Journal of Vascular Research, Vol. 49, No. 5, 01.08.2012, p. 447-460.

Research output: Contribution to journalArticle

Tréguer, K, Heinrich, EM, Ohtani, K, Bonauer, A & Dimmeler, S 2012, 'Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells', Journal of Vascular Research, vol. 49, no. 5, pp. 447-460. https://doi.org/10.1159/000339429
Tréguer, Karine ; Heinrich, Eva Marie ; Ohtani, Kisho ; Bonauer, Angelika ; Dimmeler, Stefanie. / Role of the microRNA-17-92 cluster in the endothelial differentiation of stem cells. In: Journal of Vascular Research. 2012 ; Vol. 49, No. 5. pp. 447-460.
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