Role of the osmolyte taurine on the folding of a model protein, hen egg white lysozyme, under a crowding condition

Yoshito Abe, Takatoshi Ohkuri, Sachiko Yoshitomi, Shigeru Murakami, Tadashi Ueda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Taurine is one of the osmolytes that maintain the structure of proteins in cells exposed to denaturing environmental stressors. Recently, cryoelectron tomographic analysis of eukaryotic cells has revealed that their cytoplasms are crowded with proteins. Such crowding conditions would be expected to hinder the efficient folding of nascent polypeptide chains. Therefore, we examined the role of taurine on the folding of denatured and reduced lysozyme, as a model protein, under a crowding condition. The results confirmed that taurine had a better effect on protein folding than did β-alanine, which has a similar chemical structure, when the protein to be folded was present at submillimolar concentration. NMR analyses further revealed that under the crowding condition, taurine had more interactions than did β-alanine with the lysozyme molecule in both the folded and denatured states. We concluded that taurine improves the folding of the reduced lysozyme at submillimolar concentration to allow it to interact more favorably with the lysozyme molecule. Thus, the role of taurine, as an osmolyte in vivo, may be to assist in the efficient folding of proteins.

Original languageEnglish
Pages (from-to)909-915
Number of pages7
JournalAmino Acids
Volume47
Issue number5
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Taurine
Muramidase
Proteins
Protein Folding
Alanine
Protein folding
Molecules
Eukaryotic Cells
hen egg lysozyme
Cytoplasm
Nuclear magnetic resonance
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Role of the osmolyte taurine on the folding of a model protein, hen egg white lysozyme, under a crowding condition. / Abe, Yoshito; Ohkuri, Takatoshi; Yoshitomi, Sachiko; Murakami, Shigeru; Ueda, Tadashi.

In: Amino Acids, Vol. 47, No. 5, 01.05.2015, p. 909-915.

Research output: Contribution to journalArticle

Abe, Yoshito ; Ohkuri, Takatoshi ; Yoshitomi, Sachiko ; Murakami, Shigeru ; Ueda, Tadashi. / Role of the osmolyte taurine on the folding of a model protein, hen egg white lysozyme, under a crowding condition. In: Amino Acids. 2015 ; Vol. 47, No. 5. pp. 909-915.
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