Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA

Haruna Tsukioka, Masateru Takahashi, Hiroaki Mon, Kazuhiro Okano, Kazuei Mita, Toru Shimada, Jae Man Lee, Yutaka Kawaguchi, Katsumi Koga, Takahiro Kusakabe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The argonaute protein family provides central components for RNA interference (RNAi) and related phenomena in a wide variety of organisms. Here, we isolated, from a Bombyx mori cell, a cDNA clone named BmAGO2 which is homologous to Drosophila ARGONAUTE2 the gene encoding a repressive factor for the recombination repair of extrachromosomal double-strand breaks (DSBs). RNAi-mediated silencing of the BmAGO2 sequence markedly increased homologous recombination (HR) repair of DSBs in episomal DNA, but had no effect on that in chromosomes. Moreover, we found that RNAi for BmAGO2 enhanced the integration of linearized DNA into a silkworm chromosome via HR. These results suggested that BmAgo2 protein plays an indispensable role in the repression of extrachromosomal DSB repair.

Original languageEnglish
Pages (from-to)1092-1101
Number of pages10
JournalNucleic acids research
Volume34
Issue number4
DOIs
Publication statusPublished - Mar 1 2006

Fingerprint

Bombyx
RNA Interference
DNA Repair
Recombinational DNA Repair
Argonaute Proteins
Chromosomes
Genes
Homologous Recombination
DNA
Drosophila
Complementary DNA
Clone Cells
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA. / Tsukioka, Haruna; Takahashi, Masateru; Mon, Hiroaki; Okano, Kazuhiro; Mita, Kazuei; Shimada, Toru; Lee, Jae Man; Kawaguchi, Yutaka; Koga, Katsumi; Kusakabe, Takahiro.

In: Nucleic acids research, Vol. 34, No. 4, 01.03.2006, p. 1092-1101.

Research output: Contribution to journalArticle

Tsukioka, Haruna ; Takahashi, Masateru ; Mon, Hiroaki ; Okano, Kazuhiro ; Mita, Kazuei ; Shimada, Toru ; Lee, Jae Man ; Kawaguchi, Yutaka ; Koga, Katsumi ; Kusakabe, Takahiro. / Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA. In: Nucleic acids research. 2006 ; Vol. 34, No. 4. pp. 1092-1101.
@article{183623c624134b558c0929d42c902f32,
title = "Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA",
abstract = "The argonaute protein family provides central components for RNA interference (RNAi) and related phenomena in a wide variety of organisms. Here, we isolated, from a Bombyx mori cell, a cDNA clone named BmAGO2 which is homologous to Drosophila ARGONAUTE2 the gene encoding a repressive factor for the recombination repair of extrachromosomal double-strand breaks (DSBs). RNAi-mediated silencing of the BmAGO2 sequence markedly increased homologous recombination (HR) repair of DSBs in episomal DNA, but had no effect on that in chromosomes. Moreover, we found that RNAi for BmAGO2 enhanced the integration of linearized DNA into a silkworm chromosome via HR. These results suggested that BmAgo2 protein plays an indispensable role in the repression of extrachromosomal DSB repair.",
author = "Haruna Tsukioka and Masateru Takahashi and Hiroaki Mon and Kazuhiro Okano and Kazuei Mita and Toru Shimada and Lee, {Jae Man} and Yutaka Kawaguchi and Katsumi Koga and Takahiro Kusakabe",
year = "2006",
month = "3",
day = "1",
doi = "10.1093/nar/gkj507",
language = "English",
volume = "34",
pages = "1092--1101",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA

AU - Tsukioka, Haruna

AU - Takahashi, Masateru

AU - Mon, Hiroaki

AU - Okano, Kazuhiro

AU - Mita, Kazuei

AU - Shimada, Toru

AU - Lee, Jae Man

AU - Kawaguchi, Yutaka

AU - Koga, Katsumi

AU - Kusakabe, Takahiro

PY - 2006/3/1

Y1 - 2006/3/1

N2 - The argonaute protein family provides central components for RNA interference (RNAi) and related phenomena in a wide variety of organisms. Here, we isolated, from a Bombyx mori cell, a cDNA clone named BmAGO2 which is homologous to Drosophila ARGONAUTE2 the gene encoding a repressive factor for the recombination repair of extrachromosomal double-strand breaks (DSBs). RNAi-mediated silencing of the BmAGO2 sequence markedly increased homologous recombination (HR) repair of DSBs in episomal DNA, but had no effect on that in chromosomes. Moreover, we found that RNAi for BmAGO2 enhanced the integration of linearized DNA into a silkworm chromosome via HR. These results suggested that BmAgo2 protein plays an indispensable role in the repression of extrachromosomal DSB repair.

AB - The argonaute protein family provides central components for RNA interference (RNAi) and related phenomena in a wide variety of organisms. Here, we isolated, from a Bombyx mori cell, a cDNA clone named BmAGO2 which is homologous to Drosophila ARGONAUTE2 the gene encoding a repressive factor for the recombination repair of extrachromosomal double-strand breaks (DSBs). RNAi-mediated silencing of the BmAGO2 sequence markedly increased homologous recombination (HR) repair of DSBs in episomal DNA, but had no effect on that in chromosomes. Moreover, we found that RNAi for BmAGO2 enhanced the integration of linearized DNA into a silkworm chromosome via HR. These results suggested that BmAgo2 protein plays an indispensable role in the repression of extrachromosomal DSB repair.

UR - http://www.scopus.com/inward/record.url?scp=33644859157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644859157&partnerID=8YFLogxK

U2 - 10.1093/nar/gkj507

DO - 10.1093/nar/gkj507

M3 - Article

C2 - 16478716

AN - SCOPUS:33644859157

VL - 34

SP - 1092

EP - 1101

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 4

ER -