Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA

Haruna Tsukioka, Masateru Takahashi, Hiroaki Mon, Kazuhiro Okano, Kazuei Mita, Toru Shimada, Jae Man Lee, Yutaka Kawaguchi, Katsumi Koga, Takahiro Kusakabe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The argonaute protein family provides central components for RNA interference (RNAi) and related phenomena in a wide variety of organisms. Here, we isolated, from a Bombyx mori cell, a cDNA clone named BmAGO2 which is homologous to Drosophila ARGONAUTE2 the gene encoding a repressive factor for the recombination repair of extrachromosomal double-strand breaks (DSBs). RNAi-mediated silencing of the BmAGO2 sequence markedly increased homologous recombination (HR) repair of DSBs in episomal DNA, but had no effect on that in chromosomes. Moreover, we found that RNAi for BmAGO2 enhanced the integration of linearized DNA into a silkworm chromosome via HR. These results suggested that BmAgo2 protein plays an indispensable role in the repression of extrachromosomal DSB repair.

Original languageEnglish
Pages (from-to)1092-1101
Number of pages10
JournalNucleic acids research
Volume34
Issue number4
DOIs
Publication statusPublished - Mar 2006

All Science Journal Classification (ASJC) codes

  • Genetics

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