Role of transforming growth factor-β1 in invasion and metastasis in gastric carcinoma

Yoshihiko Maehara, Yoshihiro Kakeji, Akira Kabashima, Yasunori Emi, Akihiro Watanabe, Kohei Akazawa, Hideo Baba, Shunji Kohnoe, Keizo Sugimachi

Research output: Contribution to journalArticlepeer-review

148 Citations (Scopus)


Purpose: Transforming growth factor-beta1 (TGF-β1) is a major modulator of cellular proliferation and extracellular matrix formation. We determined the role of TGF-β1 in invasion and metastasis in gastric cancer. Materials and Methods: We detected TGF-β1 expression in primary and lymph node metastatic lesions of gastric cancer, using an antibody and in situ hybridization. The plasma TGF-β1 levels in the peripheral vein and in the tumor drainage vein were assayed. Results: In the cytoplasm of cancer cells, TGF-β1 was immunostained in 35.9% (78 of 217) of primary gastric carcinomas, and this expression was confirmed by in situ hybridization. Of 59 gastric carcinomas with a TGF-β1-negative primary tumor, metastatic lymph nodes were positive for TGF-β1 staining in 32 cases (54.2%). Positive staining of TGF- β1 in gastric cancer tissues was closely related to serosal invasion, infiltrative growth, and lymph node metastasis. Multivariate analysis showed that the expression of TGF-β1 was an independent risk factor for serosal invasion and infiltrative growth of the tumor. The plasma level of TGF-β1 did not differ between TGF-β1-negative and -positive groups. There were also no differences in plasma TGF-β1 levels among each tumor stage, between the peripheral and the tumor drainage veins, and between preoperative and postoperative testings. Conclusion: Transforming growth factor-β1 is closely related to the invasion and metastasis of gastric cancer, and production of TGF-β1 in the tumor does not contribute to the total amount of TGF-β1 in the blood circulation. We interpret our observations to mean that in a tumor microenvironment, TGF-β1 alters the biologic behavior of the tumor.

Original languageEnglish
Pages (from-to)607-614
Number of pages8
JournalJournal of Clinical Oncology
Issue number2
Publication statusPublished - Feb 1999

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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