Role of tumour necrosis factor-α (TNFα) in the functional properties of hyalocytes

Yasuaki Hata, Shintaro Nakao, Ri Ichiro Kohno, Kumiyo Oba, Takeshi Kita, Muneki Miura, Yukio Sassa, Alexander Schering, Tatsuro Ishibashi

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7 Citations (Scopus)

Abstract

Background/aim: Tumour necrosis factor-α (TNFα) is an inflammatory cytokine that is upregulated in various vitreoretinal diseases including uveitis and diabetic retinopathy. Recently, our studies have indicated that hyalocytes contribute to the pathogenesis of these diseases. However, the impact of TNFα on the functional properties of hyalocytes is unknown. Methods: Hyalocytes were isolated from bovine eyes. Cellular proliferation, migration and gel contraction in response to TNFα and the other inflammatory cytokines were analysed by thymidine uptake, Boyden's chamber assay and collagen gel contraction assay, respectively. Furthermore, we estimated the effect of dexamethasone on these properties of hyalocytes. Results: TNFα promoted proliferation, migration and gel contraction by hyalocytes. Dexamethasone inhibited TNFα-induced proliferation but not migration. Dexamethasone did not inhibit TNFα-induced gel contraction but further increased contraction. Furthermore, dexamethasone inhibited TNFα-induced extracellular signal-related kinase (ERK)1/2 phosphorylation in hyalocytes. Conclusion: This study indicates that TNFα in vitreous and retina causes activation of hyalocytes, and the activated hyalocytes contribute to the pathogenesis of inflammatory vitreoretinal diseases. Steroid treatment appears to inhibit the activation of hyalocytes in the early stages of the diseases, but might have adverse effects in the late stage through membrane contraction.

Original languageEnglish
Pages (from-to)261-265
Number of pages5
JournalBritish Journal of Ophthalmology
Volume95
Issue number2
DOIs
Publication statusPublished - Feb 2011

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Hata, Y., Nakao, S., Kohno, R. I., Oba, K., Kita, T., Miura, M., Sassa, Y., Schering, A., & Ishibashi, T. (2011). Role of tumour necrosis factor-α (TNFα) in the functional properties of hyalocytes. British Journal of Ophthalmology, 95(2), 261-265. https://doi.org/10.1136/bjo.2010.190322