Role of UCP2 expression after hepatic warm ischemia-reperfusion in the rat

Mizuki Ninomiya, Ken Shirabe, Mitsuo Shimada, Takahiro Terashi, Yoshihiko Maehara

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background/Aims: The role of uncoupling protein-2 (UCP2) in the liver is currently unclear. Emerging evidence suggests a relationship between UCP2 and oxidative stress. In the present study, we tested the hypothesis that UCP2 expression in the liver might change during warm ischemia-reperfusion (I/R) according to oxidative stress. Methods: Wistar rats were subjected to 40 (short ischemia) or 90 (long ischemia) minutes of partial lobar ischemia followed by 4 hours of reperfusion. UCP2 expression in the ischemic and nonischemic lobes was assessed using reverse transcription-polymerase chain reaction and immunohistochemistry. Malondialdehyde concentrations in the liver tissue were also compared. Results: Malondialdehyde concentrations in the ischemic lobes were significantly higher in the long ischemia group. In the ischemic lobes of the short ischemia group, UCP2 protein expression was induced in hepatocytes, which did not express the protein prior to treatment, and the expression levels were higher than in the long ischemia group. The intralobular distribution of UCP2 seemed to correlate inversely with that of the necrotic area. UCP2 expression was observed, even in nonischemic lobes with similar intralobular heterogeneity. Conclusions: UCP2 was induced in hepatocytes after warm I/R. Although the primitive role of UCP2 expression may be cytoprotective in nature, its actual protective effect in hepatic I/R may be minimal.

Original languageEnglish
Pages (from-to)486-492
Number of pages7
JournalGut and Liver
Volume5
Issue number4
DOIs
Publication statusPublished - Dec 1 2011

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Warm Ischemia
Reperfusion
Ischemia
Liver
Malondialdehyde
Hepatocytes
Oxidative Stress
Uncoupling Protein 2
Reverse Transcription
Wistar Rats
Proteins
Immunohistochemistry
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Ninomiya, M., Shirabe, K., Shimada, M., Terashi, T., & Maehara, Y. (2011). Role of UCP2 expression after hepatic warm ischemia-reperfusion in the rat. Gut and Liver, 5(4), 486-492. https://doi.org/10.5009/gnl.2011.5.4.486

Role of UCP2 expression after hepatic warm ischemia-reperfusion in the rat. / Ninomiya, Mizuki; Shirabe, Ken; Shimada, Mitsuo; Terashi, Takahiro; Maehara, Yoshihiko.

In: Gut and Liver, Vol. 5, No. 4, 01.12.2011, p. 486-492.

Research output: Contribution to journalArticle

Ninomiya, M, Shirabe, K, Shimada, M, Terashi, T & Maehara, Y 2011, 'Role of UCP2 expression after hepatic warm ischemia-reperfusion in the rat', Gut and Liver, vol. 5, no. 4, pp. 486-492. https://doi.org/10.5009/gnl.2011.5.4.486
Ninomiya M, Shirabe K, Shimada M, Terashi T, Maehara Y. Role of UCP2 expression after hepatic warm ischemia-reperfusion in the rat. Gut and Liver. 2011 Dec 1;5(4):486-492. https://doi.org/10.5009/gnl.2011.5.4.486
Ninomiya, Mizuki ; Shirabe, Ken ; Shimada, Mitsuo ; Terashi, Takahiro ; Maehara, Yoshihiko. / Role of UCP2 expression after hepatic warm ischemia-reperfusion in the rat. In: Gut and Liver. 2011 ; Vol. 5, No. 4. pp. 486-492.
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