Roles of Ca 2+ on the inositol 1,4,5-trisphosphate-induced release of Ca 2+ from saponin-permeabilized single cells of the porcine coronary artery

Suematsu Eiichi, Hirata Masato, Toshiyuki Sasaguri, Hashimoto Toshihiko, Kuriyama Hirosi

Research output: Contribution to journalArticle

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Abstract

1. 1. The release of Ca 2+ from the intracellular store site, as induced by inositol 1,4,5-trisphosphate, was studied in relation to free Ca 2+ concentrations or amounts of stored Ca 2+ in smooth muscle cells. 2. 2. The maximal Ca 2+ release induced by inositol 1,4,5-trisphosphate was observed when the amount of Ca 2+ in the store site was about 50% of the maximal capacity of the Ca 2+ storage, and when the extra-vesicular free Ca 2+ concentration was less than 1.5 × 10 -6 M. 3. 3. The Ca 2+ release induced by inositol 1,4,5-trisphosphate was accelerated by ATP and 5'-adenylylimidodiphosphate (AMPPNP), but not by ADP and AMP. 4. 4. This inositol 1,4,5-trisphosphate-induced Ca 2+ release appeared to be specific for intracellular Ca 2+ store sites (mainly sarcoplasmic reticulum), and this Ca 2+ release was not apparent in the sarcolemmal fraction.

Original languageEnglish
Pages (from-to)645-649
Number of pages5
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume82
Issue number3
DOIs
Publication statusPublished - Jan 1 1985
Externally publishedYes

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Inositol 1,4,5-Trisphosphate
Saponins
Coronary Vessels
Swine
Adenylyl Imidodiphosphate
Sarcoplasmic Reticulum
Adenosine Monophosphate
Adenosine Diphosphate
Smooth Muscle Myocytes
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Roles of Ca 2+ on the inositol 1,4,5-trisphosphate-induced release of Ca 2+ from saponin-permeabilized single cells of the porcine coronary artery . / Eiichi, Suematsu; Masato, Hirata; Sasaguri, Toshiyuki; Toshihiko, Hashimoto; Hirosi, Kuriyama.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 82, No. 3, 01.01.1985, p. 645-649.

Research output: Contribution to journalArticle

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