Roles of calcitonin gene-related peptide in facilitation of wound healing and angiogenesis

Masaya Toda, Tatsunori Suzuki, Kanako Hosono, Yukiko Kurihara, Hiroki Kurihara, Izumi Hayashi, Hedero Kitasato, Sumio Hoka, Masataka Majima

Research output: Contribution to journalArticle

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Abstract

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide produced by tissue-specific alternative splicing of the primary transcript of the calcitonin/CGRP gene. CGRP is widely distributed in the central and peripheral neuronal systems and exhibits numerous biological activities in mammals. We examined in the present study whether or not endogenous CGRP released from neuronal systems facilitates neovascularization indispensable to wound healing. In CGRP knockout mice (CGRP-/-), wound-induced angiogenesis and wound closure were significantly suppressed compared with those in wild-type mice. The suppressed healing in CGRP-/- was accompanied by reduction in expressions of vascular endothelial growth factor (VEGF) in the wound granulation tissues. A CGRP antagonist, CGRP8-37 when infused with mini-osmotic pumps subcutaneously blocked the wound healing processes and reduced the expressions of CD31 and VEGF expression in the wound granulation tissues. Wound healing process was significantly delayed in neuropeptide-depleted mice pretreated with capsaicin, compared with vehicle-treated mice. These results indicate that CGRP derived from neuronal systems may facilitate wound healing and angiogenesis. Targeting of CGRP may be promising in controlling angiogenesis related to pathophysiological conditions.

Original languageEnglish
Pages (from-to)352-359
Number of pages8
JournalBiomedicine and Pharmacotherapy
Volume62
Issue number6
DOIs
Publication statusPublished - Jul 1 2008

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Calcitonin Gene-Related Peptide
Wound Healing
Granulation Tissue
Wounds and Injuries
Neuropeptides
Vascular Endothelial Growth Factor A
Capsaicin
Alternative Splicing
Calcitonin
Knockout Mice
Mammals
Amino Acids

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Toda, M., Suzuki, T., Hosono, K., Kurihara, Y., Kurihara, H., Hayashi, I., ... Majima, M. (2008). Roles of calcitonin gene-related peptide in facilitation of wound healing and angiogenesis. Biomedicine and Pharmacotherapy, 62(6), 352-359. https://doi.org/10.1016/j.biopha.2008.02.003

Roles of calcitonin gene-related peptide in facilitation of wound healing and angiogenesis. / Toda, Masaya; Suzuki, Tatsunori; Hosono, Kanako; Kurihara, Yukiko; Kurihara, Hiroki; Hayashi, Izumi; Kitasato, Hedero; Hoka, Sumio; Majima, Masataka.

In: Biomedicine and Pharmacotherapy, Vol. 62, No. 6, 01.07.2008, p. 352-359.

Research output: Contribution to journalArticle

Toda, M, Suzuki, T, Hosono, K, Kurihara, Y, Kurihara, H, Hayashi, I, Kitasato, H, Hoka, S & Majima, M 2008, 'Roles of calcitonin gene-related peptide in facilitation of wound healing and angiogenesis', Biomedicine and Pharmacotherapy, vol. 62, no. 6, pp. 352-359. https://doi.org/10.1016/j.biopha.2008.02.003
Toda, Masaya ; Suzuki, Tatsunori ; Hosono, Kanako ; Kurihara, Yukiko ; Kurihara, Hiroki ; Hayashi, Izumi ; Kitasato, Hedero ; Hoka, Sumio ; Majima, Masataka. / Roles of calcitonin gene-related peptide in facilitation of wound healing and angiogenesis. In: Biomedicine and Pharmacotherapy. 2008 ; Vol. 62, No. 6. pp. 352-359.
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