Roles of hydroxyl radical generating/scavenging mechanisms in pseudo polluted dew in reducing the foliar CO2 assimilation rate and biomass production of Japanese red pine (Pinus densiflora Sieb. et Zucc.) seedlings

Nobutake Nakatani, Sachiko Akane, Masaaki Chiwa, Tsuyoshi Kobayashi, Hiroshi Sakugawa

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Abstract

Hydroxyl radical ({radical dot}OH) is one of the most highly reactive of all active oxygen species. Aqueous-phase {radical dot}OH is photochemically generated in polluted dew droplets on needle surfaces of Japanese red pine (Pinus densiflora Sieb. et Zucc., an evergreen coniferous tree) in declining pine forests. In former studies, we examined the effects of {radical dot}OH-generating mist solutions, which simulate polluted dew droplets, on needle ecophysiological traits of pine seedlings. Two types of solution with different {radical dot}OH-generating sources, i.e. the photo-Fenton reaction (H2O2-Fe-oxalate) and photolysis of HONO and NO2- [N(III)], had contrasting functional effects on needle gas exchange characteristics even though they had similar {radical dot}OH photoformation rates. In the present study, we investigated the effects of {radical dot}OH-generating mist solutions containing mixed-sources of {radical dot}OH (photo-Fenton and N(III)) on needle gas exchange and the biomass production of potted Japanese red pine seedlings. The N(III) and H2O2 concentrations of the {radical dot}OH-generating mist solutions were regulated to the same concentration (0, 25, 50, 75 or 100 μM, pH 5.2-5.3). Treated needles with the lowest photoformation rate and scavenging rate constant of {radical dot}OH had the smallest CO2 assimilation rate (Amax) and needle conductance (gn) of all treated pine seedlings. This suggests that the photoformation rate and scavenging rate constant do not always explain the ecophysiological disorders of pine needles subjected to {radical dot}OH-generating wet deposition. On the other hand, the calculated steady-state concentration of {radical dot}OH in the mist solutions was significantly negatively correlated with Amax. In addition, pine seedlings with reduced Amax showed suppressed biomass (dry weight) production. These results suggest that the {radical dot}OH concentration in the mist solutions is the decisive factor in explaining the negative effects of pseudo dew droplets not only on needle but also plant productivity. We propose that the deposition of polluted dew with chemical components and systems that result in a high {radical dot}OH concentration is one of the causes of growth decline of Japanese red pine.

Original languageEnglish
Pages (from-to)159-169
Number of pages11
JournalEnvironmental and Experimental Botany
Volume60
Issue number2
DOIs
Publication statusPublished - Jun 1 2007
Externally publishedYes

Fingerprint

Pinus densiflora
dew
hydroxyl radicals
hydroxyl radical
assimilation (physiology)
biomass production
seedling
seedlings
biomass
droplets
droplet
Pinus
gas exchange
rate
assimilation
wet deposition
evergreen tree
conifer needles
photolysis
oxalates

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{1c8f49320ba442908a4c91bb794a3d46,
title = "Roles of hydroxyl radical generating/scavenging mechanisms in pseudo polluted dew in reducing the foliar CO2 assimilation rate and biomass production of Japanese red pine (Pinus densiflora Sieb. et Zucc.) seedlings",
abstract = "Hydroxyl radical ({radical dot}OH) is one of the most highly reactive of all active oxygen species. Aqueous-phase {radical dot}OH is photochemically generated in polluted dew droplets on needle surfaces of Japanese red pine (Pinus densiflora Sieb. et Zucc., an evergreen coniferous tree) in declining pine forests. In former studies, we examined the effects of {radical dot}OH-generating mist solutions, which simulate polluted dew droplets, on needle ecophysiological traits of pine seedlings. Two types of solution with different {radical dot}OH-generating sources, i.e. the photo-Fenton reaction (H2O2-Fe-oxalate) and photolysis of HONO and NO2- [N(III)], had contrasting functional effects on needle gas exchange characteristics even though they had similar {radical dot}OH photoformation rates. In the present study, we investigated the effects of {radical dot}OH-generating mist solutions containing mixed-sources of {radical dot}OH (photo-Fenton and N(III)) on needle gas exchange and the biomass production of potted Japanese red pine seedlings. The N(III) and H2O2 concentrations of the {radical dot}OH-generating mist solutions were regulated to the same concentration (0, 25, 50, 75 or 100 μM, pH 5.2-5.3). Treated needles with the lowest photoformation rate and scavenging rate constant of {radical dot}OH had the smallest CO2 assimilation rate (Amax) and needle conductance (gn) of all treated pine seedlings. This suggests that the photoformation rate and scavenging rate constant do not always explain the ecophysiological disorders of pine needles subjected to {radical dot}OH-generating wet deposition. On the other hand, the calculated steady-state concentration of {radical dot}OH in the mist solutions was significantly negatively correlated with Amax. In addition, pine seedlings with reduced Amax showed suppressed biomass (dry weight) production. These results suggest that the {radical dot}OH concentration in the mist solutions is the decisive factor in explaining the negative effects of pseudo dew droplets not only on needle but also plant productivity. We propose that the deposition of polluted dew with chemical components and systems that result in a high {radical dot}OH concentration is one of the causes of growth decline of Japanese red pine.",
author = "Nobutake Nakatani and Sachiko Akane and Masaaki Chiwa and Tsuyoshi Kobayashi and Hiroshi Sakugawa",
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T1 - Roles of hydroxyl radical generating/scavenging mechanisms in pseudo polluted dew in reducing the foliar CO2 assimilation rate and biomass production of Japanese red pine (Pinus densiflora Sieb. et Zucc.) seedlings

AU - Nakatani, Nobutake

AU - Akane, Sachiko

AU - Chiwa, Masaaki

AU - Kobayashi, Tsuyoshi

AU - Sakugawa, Hiroshi

PY - 2007/6/1

Y1 - 2007/6/1

N2 - Hydroxyl radical ({radical dot}OH) is one of the most highly reactive of all active oxygen species. Aqueous-phase {radical dot}OH is photochemically generated in polluted dew droplets on needle surfaces of Japanese red pine (Pinus densiflora Sieb. et Zucc., an evergreen coniferous tree) in declining pine forests. In former studies, we examined the effects of {radical dot}OH-generating mist solutions, which simulate polluted dew droplets, on needle ecophysiological traits of pine seedlings. Two types of solution with different {radical dot}OH-generating sources, i.e. the photo-Fenton reaction (H2O2-Fe-oxalate) and photolysis of HONO and NO2- [N(III)], had contrasting functional effects on needle gas exchange characteristics even though they had similar {radical dot}OH photoformation rates. In the present study, we investigated the effects of {radical dot}OH-generating mist solutions containing mixed-sources of {radical dot}OH (photo-Fenton and N(III)) on needle gas exchange and the biomass production of potted Japanese red pine seedlings. The N(III) and H2O2 concentrations of the {radical dot}OH-generating mist solutions were regulated to the same concentration (0, 25, 50, 75 or 100 μM, pH 5.2-5.3). Treated needles with the lowest photoformation rate and scavenging rate constant of {radical dot}OH had the smallest CO2 assimilation rate (Amax) and needle conductance (gn) of all treated pine seedlings. This suggests that the photoformation rate and scavenging rate constant do not always explain the ecophysiological disorders of pine needles subjected to {radical dot}OH-generating wet deposition. On the other hand, the calculated steady-state concentration of {radical dot}OH in the mist solutions was significantly negatively correlated with Amax. In addition, pine seedlings with reduced Amax showed suppressed biomass (dry weight) production. These results suggest that the {radical dot}OH concentration in the mist solutions is the decisive factor in explaining the negative effects of pseudo dew droplets not only on needle but also plant productivity. We propose that the deposition of polluted dew with chemical components and systems that result in a high {radical dot}OH concentration is one of the causes of growth decline of Japanese red pine.

AB - Hydroxyl radical ({radical dot}OH) is one of the most highly reactive of all active oxygen species. Aqueous-phase {radical dot}OH is photochemically generated in polluted dew droplets on needle surfaces of Japanese red pine (Pinus densiflora Sieb. et Zucc., an evergreen coniferous tree) in declining pine forests. In former studies, we examined the effects of {radical dot}OH-generating mist solutions, which simulate polluted dew droplets, on needle ecophysiological traits of pine seedlings. Two types of solution with different {radical dot}OH-generating sources, i.e. the photo-Fenton reaction (H2O2-Fe-oxalate) and photolysis of HONO and NO2- [N(III)], had contrasting functional effects on needle gas exchange characteristics even though they had similar {radical dot}OH photoformation rates. In the present study, we investigated the effects of {radical dot}OH-generating mist solutions containing mixed-sources of {radical dot}OH (photo-Fenton and N(III)) on needle gas exchange and the biomass production of potted Japanese red pine seedlings. The N(III) and H2O2 concentrations of the {radical dot}OH-generating mist solutions were regulated to the same concentration (0, 25, 50, 75 or 100 μM, pH 5.2-5.3). Treated needles with the lowest photoformation rate and scavenging rate constant of {radical dot}OH had the smallest CO2 assimilation rate (Amax) and needle conductance (gn) of all treated pine seedlings. This suggests that the photoformation rate and scavenging rate constant do not always explain the ecophysiological disorders of pine needles subjected to {radical dot}OH-generating wet deposition. On the other hand, the calculated steady-state concentration of {radical dot}OH in the mist solutions was significantly negatively correlated with Amax. In addition, pine seedlings with reduced Amax showed suppressed biomass (dry weight) production. These results suggest that the {radical dot}OH concentration in the mist solutions is the decisive factor in explaining the negative effects of pseudo dew droplets not only on needle but also plant productivity. We propose that the deposition of polluted dew with chemical components and systems that result in a high {radical dot}OH concentration is one of the causes of growth decline of Japanese red pine.

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