Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes

Yuka Kabayama, Hidehiro Toh, Ami Katanaya, Takayuki Sakurai, Shinichiro Chuma, Satomi Kuramochi-Miyagawa, Yumiko Saga, Toru Nakano, Hiroyuki Sasaki

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    The mouse PIWI-interacting RNA (piRNA) pathway produces a class of 26-30-nucleotide (nt) small RNAs and is essential for spermatogenesis and retrotransposon repression. In oocytes, however, its regulation and function are poorly understood. In the present study, we investigated the consequences of loss of piRNA-pathway components in growing oocytes. When MILI (or PIWIL2), a PIWI family member, was depleted by gene knockout, almost all piRNAs disappeared. This severe loss of piRNA was accompanied by an increase in transcripts derived from specific retrotransposons, especially IAPs. MIWI (or PIWIL1) depletion had a smaller effect. In oocytes lacking PLD6 (or ZUCCHINI or MITOPLD), amitochondrial nuclease/phospholipase involved in piRNA biogenesis inmale germ cells, the piRNA level was decreased to 50% compared to wild-type, a phenotype much milder than that in males. Since PLD6 is essential for the creation of the 5- ends of primary piRNAs in males, the presence of mature piRNA in PLD6-depleted oocytes suggests the presence of compensating enzymes. Furthermore, we identified novel 21-23-nt small RNAs, termed spiRNAs, possessing a 10-nt complementarity with piRNAs, which were produced dependent on MILI and independent of DICER. Our study revealed the differences in the biogenesis and function of the piRNA pathway between sexes.

    Original languageEnglish
    Pages (from-to)5387-5398
    Number of pages12
    JournalNucleic acids research
    Volume45
    Issue number9
    DOIs
    Publication statusPublished - May 19 2017

    All Science Journal Classification (ASJC) codes

    • Genetics

    Fingerprint Dive into the research topics of 'Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes'. Together they form a unique fingerprint.

  • Cite this

    Kabayama, Y., Toh, H., Katanaya, A., Sakurai, T., Chuma, S., Kuramochi-Miyagawa, S., Saga, Y., Nakano, T., & Sasaki, H. (2017). Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes. Nucleic acids research, 45(9), 5387-5398. https://doi.org/10.1093/nar/gkx027