Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-D-glucoside as the active component, stimulates exocrine glands via the M 3 muscarinic acetylcholine receptor

Saori Nishimachi, Takao Iwai, Yoshitaka Nakamura, Kanako Yuyama-Masuda, Takuya Tsutsui, Noriyuki Monoi, Akira Uchiyama, Seiji Nakamura

Research output: Contribution to journalArticle

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Abstract

We previously discovered that rooibos extract activates the M 3 muscarinic acetylcholine receptor (M3R), a key component of exocrine secretion. In this study, we examined the rooibos extract for active components in vitro and monitored its fast-acting effects against dryness in humans. Our in vitro analysis demonstrated that the M3R-stimulating effect of rooibos extract was similar to that of eriodictyol-6-C-β-D-glucoside (E6CG), whereas other rooibos constituents (aspalathin, orientin, isoorientin or eriodictyol-8-C-β-D-glucoside) were not active. In a placebo-controlled, double-blinded crossover clinical trial, 36 subjects with dryness at the mouth, eyes, and skin consumed a daily dose of either 100 mg rooibos or placebo tablets for three days. Rooibos extract ingestion increased resting-saliva content, tear volume, and stratum corneum moisture content, and reduced the dryness symptoms. In conclusion, rooibos extract contains E6CG as a M3R activator and stimulates exocrine gland secretion, which relieves dryness at the mouth, eyes, and skin in humans.

Original languageEnglish
Pages (from-to)248-254
Number of pages7
JournalJournal of Functional Foods
Volume55
DOIs
Publication statusPublished - Apr 1 2019

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Aspalathus
Exocrine Glands
exocrine glands
cholinergic receptors
Glucosides
Muscarinic Receptors
glucosides
extracts
Xerostomia
homoorientin
skin (animal)
placebos
mouth
eyes
Placebos
secretion
Skin
cornea
eriodictyol
Aspalathus linearis

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-D-glucoside as the active component, stimulates exocrine glands via the M 3 muscarinic acetylcholine receptor . / Nishimachi, Saori; Iwai, Takao; Nakamura, Yoshitaka; Yuyama-Masuda, Kanako; Tsutsui, Takuya; Monoi, Noriyuki; Uchiyama, Akira; Nakamura, Seiji.

In: Journal of Functional Foods, Vol. 55, 01.04.2019, p. 248-254.

Research output: Contribution to journalArticle

Nishimachi, Saori ; Iwai, Takao ; Nakamura, Yoshitaka ; Yuyama-Masuda, Kanako ; Tsutsui, Takuya ; Monoi, Noriyuki ; Uchiyama, Akira ; Nakamura, Seiji. / Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-D-glucoside as the active component, stimulates exocrine glands via the M 3 muscarinic acetylcholine receptor In: Journal of Functional Foods. 2019 ; Vol. 55. pp. 248-254.
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