rRNA degradation in Bombyx mori and Bombyx mandarina cells infected witv veterologous nucleopolyvedroviruses

Rina Hamajima, Chisa Yasunaga-Aoki, Masashi Iwanaga, Shigeo Imanishi, Jun Kobayashi, Kuni Sasaki, Takahiro Kusakabe, Man Lee, Hiroaki Mon, Michihiro Kobayashi, Motoko Ikeda

Research output: Contribution to journalArticle

Abstract

We previously found that rRNA of BM-N cells derived from the silkworm Bombyx mori undergoes rapid and extensive degradation through site-specific cleavage during abortive infection with nucleopolyhedroviruses (NPVs) of Autographa californica (AcMNPV), Hyphantria cunea (HycuMNPV), Spodoptera exigua and S. litura. Here, we demonstrated that rRNA degradation also occurs in Bme21 and Bm-aff3 cells, which are derived from B. mori embryo and fat body, respectively, during infection with AcMNPV. rRNA degradation in Bme21 cells was also observed following HycuMNPV infection, but was not detected in Bm-aff3 cells. We further showed that rRNA in a cell line derived from B. mandarina, an ancestor of B. mori, underwent degradation in response to cellular infection with AcMNPV and HycuMNPV. In contrast, no rRNA degradation was observed in a cell line derived from Antheraea pernyi. Taken together, these results indicate that NPV-triggered rRNA degradation represents a mechanism of innate antiviral immunity that is unique to B. mori and B. mandarina cells.

Original languageEnglish
Pages (from-to)73-77
Number of pages5
JournalJournal of Insect Biotechnology and Sericology
Volume85
Issue number3
Publication statusPublished - Jan 1 2016

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Bombyx
Bombyx mori
ribosomal RNA
Autographa californica multiple nucleopolyhedrovirus
Degradation
degradation
Nucleopolyhedrovirus
Infection
Baculoviridae
cells
infection
Cell Line
Spodoptera
Antheraea pernyi
Cells
cell lines
Hyphantria cunea
Autographa californica
Fat Body
Moths

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

Cite this

rRNA degradation in Bombyx mori and Bombyx mandarina cells infected witv veterologous nucleopolyvedroviruses. / Hamajima, Rina; Yasunaga-Aoki, Chisa; Iwanaga, Masashi; Imanishi, Shigeo; Kobayashi, Jun; Sasaki, Kuni; Kusakabe, Takahiro; Lee, Man; Mon, Hiroaki; Kobayashi, Michihiro; Ikeda, Motoko.

In: Journal of Insect Biotechnology and Sericology, Vol. 85, No. 3, 01.01.2016, p. 73-77.

Research output: Contribution to journalArticle

Hamajima, R, Yasunaga-Aoki, C, Iwanaga, M, Imanishi, S, Kobayashi, J, Sasaki, K, Kusakabe, T, Lee, M, Mon, H, Kobayashi, M & Ikeda, M 2016, 'rRNA degradation in Bombyx mori and Bombyx mandarina cells infected witv veterologous nucleopolyvedroviruses', Journal of Insect Biotechnology and Sericology, vol. 85, no. 3, pp. 73-77.
Hamajima, Rina ; Yasunaga-Aoki, Chisa ; Iwanaga, Masashi ; Imanishi, Shigeo ; Kobayashi, Jun ; Sasaki, Kuni ; Kusakabe, Takahiro ; Lee, Man ; Mon, Hiroaki ; Kobayashi, Michihiro ; Ikeda, Motoko. / rRNA degradation in Bombyx mori and Bombyx mandarina cells infected witv veterologous nucleopolyvedroviruses. In: Journal of Insect Biotechnology and Sericology. 2016 ; Vol. 85, No. 3. pp. 73-77.
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