TY - JOUR
T1 - rRNA degradation in Bombyx mori and Bombyx mandarina cells infected witv veterologous nucleopolyvedroviruses
AU - Hamajima, Rina
AU - Yasunaga-Aoki, Chisa
AU - Iwanaga, Masashi
AU - Imanishi, Shigeo
AU - Kobayashi, Jun
AU - Sasaki, Kuni
AU - Kusakabe, Takahiro
AU - Lee, Jae Man
AU - Mon, Hiroaki
AU - Kobayashi, Michihiro
AU - Ikeda, Motoko
N1 - Funding Information:
We thank Dr. T. Yaginuma and Dr. T. Niimi of the Laboratory of Sericulture and Entomoresources, Nagoya University, Japan, for their helpful discussions during this study. This work was supported in part by JSPS KAKENHI Grant Number JP26292173 and JP15J02649. RH was supported by the Research Fellowship for Young Scientists from the JSPS and the Program for Leading Graduate Schools “gIntegrative Graduate Education and Research in Green Sciences”, Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.
Publisher Copyright:
© 2016, Japanese Society of Sericultural Sciences. All rights reserved.
PY - 2016
Y1 - 2016
N2 - We previously found that rRNA of BM-N cells derived from the silkworm Bombyx mori undergoes rapid and extensive degradation through site-specific cleavage during abortive infection with nucleopolyhedroviruses (NPVs) of Autographa californica (AcMNPV), Hyphantria cunea (HycuMNPV), Spodoptera exigua and S. litura. Here, we demonstrated that rRNA degradation also occurs in Bme21 and Bm-aff3 cells, which are derived from B. mori embryo and fat body, respectively, during infection with AcMNPV. rRNA degradation in Bme21 cells was also observed following HycuMNPV infection, but was not detected in Bm-aff3 cells. We further showed that rRNA in a cell line derived from B. mandarina, an ancestor of B. mori, underwent degradation in response to cellular infection with AcMNPV and HycuMNPV. In contrast, no rRNA degradation was observed in a cell line derived from Antheraea pernyi. Taken together, these results indicate that NPV-triggered rRNA degradation represents a mechanism of innate antiviral immunity that is unique to B. mori and B. mandarina cells.
AB - We previously found that rRNA of BM-N cells derived from the silkworm Bombyx mori undergoes rapid and extensive degradation through site-specific cleavage during abortive infection with nucleopolyhedroviruses (NPVs) of Autographa californica (AcMNPV), Hyphantria cunea (HycuMNPV), Spodoptera exigua and S. litura. Here, we demonstrated that rRNA degradation also occurs in Bme21 and Bm-aff3 cells, which are derived from B. mori embryo and fat body, respectively, during infection with AcMNPV. rRNA degradation in Bme21 cells was also observed following HycuMNPV infection, but was not detected in Bm-aff3 cells. We further showed that rRNA in a cell line derived from B. mandarina, an ancestor of B. mori, underwent degradation in response to cellular infection with AcMNPV and HycuMNPV. In contrast, no rRNA degradation was observed in a cell line derived from Antheraea pernyi. Taken together, these results indicate that NPV-triggered rRNA degradation represents a mechanism of innate antiviral immunity that is unique to B. mori and B. mandarina cells.
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M3 - Article
AN - SCOPUS:85009812015
SN - 1346-8073
VL - 85
SP - 73
EP - 77
JO - Journal of Insect Biotechnology and Sericology
JF - Journal of Insect Biotechnology and Sericology
IS - 3
ER -