S100A4 mRNA is a diagnostic and prognostic marker in pancreatic carcinoma

Naoki Ikenaga, Kenoki Ouchida, Kazuhiro Mizumoto, Jun Yu, Hayato Fujita, Kohei Nakata, Junji Ueda, Norihiro Sato, Eishi Nagai, Masao Tanaka

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Abstract

Objective The aim of this study is to evaluate the clinical significance of S100A4 mRNA expression in pancreatic cancer. Materials and Methods We obtained invasive ductal carcinoma (IDC) cells from ten lesions, intraductal papillary mucinous neoplasm (IPMN) cells from 20 lesions, and normal ductal cells from 20 normal pancreatic tissues by laser microdissection of frozen tissues. S100A4 expression was examined in the microdissected cells and in formalin-fixed paraffin-embedded (FFPE) samples of 87 pancreatic cancers by quantitative reverse transcription-polymerase chain reaction. Results IDC cells expressed higher levels of S100A4 than IPMN cells (P = 0.002) and normal ductal cells (P < 0.001), although the difference between IPMN cells and normal ductal cells was not statistically significant (P = 0.070). Analysis of FFPE samples revealed that high S100A4 expression was significantly associated with a shorter overall survival (P = 0.023). In immunohistochemical analysis, the extent of S100A4 mRNA expression was significantly correlated with the expression of S100A4 protein (P = 0.028). Conclusion S100A4 could be a marker for malignancy in pancreatic tumors and for poor prognosis in patients with pancreatic cancer.

Original languageEnglish
Pages (from-to)1852-1858
Number of pages7
JournalJournal of Gastrointestinal Surgery
Volume13
Issue number10
DOIs
Publication statusPublished - Sep 1 2009

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Messenger RNA
Pancreatic Neoplasms
Ductal Carcinoma
Neoplasms
Paraffin
Formaldehyde
Pancreatic Carcinoma
Microdissection
Reverse Transcription
Lasers
Polymerase Chain Reaction
Survival

All Science Journal Classification (ASJC) codes

  • Surgery
  • Gastroenterology

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S100A4 mRNA is a diagnostic and prognostic marker in pancreatic carcinoma. / Ikenaga, Naoki; Ouchida, Kenoki; Mizumoto, Kazuhiro; Yu, Jun; Fujita, Hayato; Nakata, Kohei; Ueda, Junji; Sato, Norihiro; Nagai, Eishi; Tanaka, Masao.

In: Journal of Gastrointestinal Surgery, Vol. 13, No. 10, 01.09.2009, p. 1852-1858.

Research output: Contribution to journalArticle

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abstract = "Objective The aim of this study is to evaluate the clinical significance of S100A4 mRNA expression in pancreatic cancer. Materials and Methods We obtained invasive ductal carcinoma (IDC) cells from ten lesions, intraductal papillary mucinous neoplasm (IPMN) cells from 20 lesions, and normal ductal cells from 20 normal pancreatic tissues by laser microdissection of frozen tissues. S100A4 expression was examined in the microdissected cells and in formalin-fixed paraffin-embedded (FFPE) samples of 87 pancreatic cancers by quantitative reverse transcription-polymerase chain reaction. Results IDC cells expressed higher levels of S100A4 than IPMN cells (P = 0.002) and normal ductal cells (P < 0.001), although the difference between IPMN cells and normal ductal cells was not statistically significant (P = 0.070). Analysis of FFPE samples revealed that high S100A4 expression was significantly associated with a shorter overall survival (P = 0.023). In immunohistochemical analysis, the extent of S100A4 mRNA expression was significantly correlated with the expression of S100A4 protein (P = 0.028). Conclusion S100A4 could be a marker for malignancy in pancreatic tumors and for poor prognosis in patients with pancreatic cancer.",
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T1 - S100A4 mRNA is a diagnostic and prognostic marker in pancreatic carcinoma

AU - Ikenaga, Naoki

AU - Ouchida, Kenoki

AU - Mizumoto, Kazuhiro

AU - Yu, Jun

AU - Fujita, Hayato

AU - Nakata, Kohei

AU - Ueda, Junji

AU - Sato, Norihiro

AU - Nagai, Eishi

AU - Tanaka, Masao

PY - 2009/9/1

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N2 - Objective The aim of this study is to evaluate the clinical significance of S100A4 mRNA expression in pancreatic cancer. Materials and Methods We obtained invasive ductal carcinoma (IDC) cells from ten lesions, intraductal papillary mucinous neoplasm (IPMN) cells from 20 lesions, and normal ductal cells from 20 normal pancreatic tissues by laser microdissection of frozen tissues. S100A4 expression was examined in the microdissected cells and in formalin-fixed paraffin-embedded (FFPE) samples of 87 pancreatic cancers by quantitative reverse transcription-polymerase chain reaction. Results IDC cells expressed higher levels of S100A4 than IPMN cells (P = 0.002) and normal ductal cells (P < 0.001), although the difference between IPMN cells and normal ductal cells was not statistically significant (P = 0.070). Analysis of FFPE samples revealed that high S100A4 expression was significantly associated with a shorter overall survival (P = 0.023). In immunohistochemical analysis, the extent of S100A4 mRNA expression was significantly correlated with the expression of S100A4 protein (P = 0.028). Conclusion S100A4 could be a marker for malignancy in pancreatic tumors and for poor prognosis in patients with pancreatic cancer.

AB - Objective The aim of this study is to evaluate the clinical significance of S100A4 mRNA expression in pancreatic cancer. Materials and Methods We obtained invasive ductal carcinoma (IDC) cells from ten lesions, intraductal papillary mucinous neoplasm (IPMN) cells from 20 lesions, and normal ductal cells from 20 normal pancreatic tissues by laser microdissection of frozen tissues. S100A4 expression was examined in the microdissected cells and in formalin-fixed paraffin-embedded (FFPE) samples of 87 pancreatic cancers by quantitative reverse transcription-polymerase chain reaction. Results IDC cells expressed higher levels of S100A4 than IPMN cells (P = 0.002) and normal ductal cells (P < 0.001), although the difference between IPMN cells and normal ductal cells was not statistically significant (P = 0.070). Analysis of FFPE samples revealed that high S100A4 expression was significantly associated with a shorter overall survival (P = 0.023). In immunohistochemical analysis, the extent of S100A4 mRNA expression was significantly correlated with the expression of S100A4 protein (P = 0.028). Conclusion S100A4 could be a marker for malignancy in pancreatic tumors and for poor prognosis in patients with pancreatic cancer.

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