Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p

Yonggang Wang, Nobutaka Nakashima, Takeshi Sekiguchi, Takeharu Nishimoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A Gtr1p GTPase, the GDP mutant of which suppresses both temperature-sensitive mutants of Saccharomyces cerevisiae RanGEF/Prp20p and RanGAP/Rna1p, was presently found to interact with Yrb2p, the S. cerevisiae homologue of mammalian Ran-binding protein 3. Gtr1p bound the Ran-binding domain of Yrb2p. In contrast, Gtr2p, a partner of Gtr1p, did not bind Yrb2p, although it bound Gtr1p. A triple mutant: yrb2Δ gtr1Δ gtr2Δ was lethal, while a double mutant: gtr1Δ gtr2Δ survived well, indicating that Yrb2p protected cells from the killing effect of gtr1Δ gtr2Δ. Recombinant Gtr1p and Gtr2p were purified as a complex from Escherichia coli. The resulting Gtr1p-Gtr2p complex was comprised of an equal amount of Gtr1p and Gtr2p, which inhibited the Rna1p/Yrb2 dependent RanGAP activity. Thus, the Gtr1p-Gtr2p cycle was suggested to regulate the Ran cycle through Yrb2p.

Original languageEnglish
Pages (from-to)639-645
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume336
Issue number2
DOIs
Publication statusPublished - Oct 21 2005

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ran GTP-Binding Protein
Saccharomyces cerevisiae Proteins
GTP Phosphohydrolases
Cell proliferation
Yeast
Saccharomyces cerevisiae
Carrier Proteins
Cell Proliferation
Escherichia coli
Temperature

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Saccharomyces cerevisiae GTPase complex : Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p. / Wang, Yonggang; Nakashima, Nobutaka; Sekiguchi, Takeshi; Nishimoto, Takeharu.

In: Biochemical and Biophysical Research Communications, Vol. 336, No. 2, 21.10.2005, p. 639-645.

Research output: Contribution to journalArticle

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