Abstract
Objectives: The aim of this study is to investigate the salicylate-induced morphological changes of cochlear inner hair cells (IHCs) and outer hair cells (OHCs). Methods: IHCs and OHCs were acutely isolated from the guinea-pig cochlea. Cells were observed under the inverted microscope and 10 mmol/L sodium salicylate solutions or 0.01 mmol/L dexamethasone-plus-salicylate solutions were applied. The cell length or the ratio between the length and width was the indices of the morphological changes in cells. Results: Isolated IHCs did not demonstrate any significant changes in sodium salicylate solutions in 20 min and in 40 min, whereas OHCs were shortened by the 10 mmol/L sodium salicylate to 83% in 20 min and 75% in 40 min. There were no significant differences between in the dexamethasone-plus-salicylate solutions and in the control solutions after 20 min and 40 min both in IHCs and OHCs. Conclusions: Although salicylate affected the isolated OHCs from guinea-pig cochlea, IHCs were not changed morphologically by sodium salicylate applications. Dexamethasone inhibited the salicylate-induced morphological changes of OHCs.
| Original language | English |
|---|---|
| Pages (from-to) | 152-156 |
| Number of pages | 5 |
| Journal | Auris Nasus Larynx |
| Volume | 36 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 1 2009 |
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All Science Journal Classification (ASJC) codes
- Surgery
- Otorhinolaryngology
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Salicylate-induced morphological changes of isolated inner hair cells and outer hair cells from guinea-pig cochlea. / Kimitsuki, Takashi; Kakazu, Yasuhiro; Matsumoto, Nozomu; Noda, Teppei; Komune, Noritaka; Komune, Shizuo.
In: Auris Nasus Larynx, Vol. 36, No. 2, 01.04.2009, p. 152-156.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Salicylate-induced morphological changes of isolated inner hair cells and outer hair cells from guinea-pig cochlea
AU - Kimitsuki, Takashi
AU - Kakazu, Yasuhiro
AU - Matsumoto, Nozomu
AU - Noda, Teppei
AU - Komune, Noritaka
AU - Komune, Shizuo
PY - 2009/4/1
Y1 - 2009/4/1
N2 - Objectives: The aim of this study is to investigate the salicylate-induced morphological changes of cochlear inner hair cells (IHCs) and outer hair cells (OHCs). Methods: IHCs and OHCs were acutely isolated from the guinea-pig cochlea. Cells were observed under the inverted microscope and 10 mmol/L sodium salicylate solutions or 0.01 mmol/L dexamethasone-plus-salicylate solutions were applied. The cell length or the ratio between the length and width was the indices of the morphological changes in cells. Results: Isolated IHCs did not demonstrate any significant changes in sodium salicylate solutions in 20 min and in 40 min, whereas OHCs were shortened by the 10 mmol/L sodium salicylate to 83% in 20 min and 75% in 40 min. There were no significant differences between in the dexamethasone-plus-salicylate solutions and in the control solutions after 20 min and 40 min both in IHCs and OHCs. Conclusions: Although salicylate affected the isolated OHCs from guinea-pig cochlea, IHCs were not changed morphologically by sodium salicylate applications. Dexamethasone inhibited the salicylate-induced morphological changes of OHCs.
AB - Objectives: The aim of this study is to investigate the salicylate-induced morphological changes of cochlear inner hair cells (IHCs) and outer hair cells (OHCs). Methods: IHCs and OHCs were acutely isolated from the guinea-pig cochlea. Cells were observed under the inverted microscope and 10 mmol/L sodium salicylate solutions or 0.01 mmol/L dexamethasone-plus-salicylate solutions were applied. The cell length or the ratio between the length and width was the indices of the morphological changes in cells. Results: Isolated IHCs did not demonstrate any significant changes in sodium salicylate solutions in 20 min and in 40 min, whereas OHCs were shortened by the 10 mmol/L sodium salicylate to 83% in 20 min and 75% in 40 min. There were no significant differences between in the dexamethasone-plus-salicylate solutions and in the control solutions after 20 min and 40 min both in IHCs and OHCs. Conclusions: Although salicylate affected the isolated OHCs from guinea-pig cochlea, IHCs were not changed morphologically by sodium salicylate applications. Dexamethasone inhibited the salicylate-induced morphological changes of OHCs.
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UR - http://www.scopus.com/inward/citedby.url?scp=59849121292&partnerID=8YFLogxK
U2 - 10.1016/j.anl.2008.05.005
DO - 10.1016/j.anl.2008.05.005
M3 - Article
C2 - 18606508
AN - SCOPUS:59849121292
VL - 36
SP - 152
EP - 156
JO - Auris Nasus Larynx
JF - Auris Nasus Larynx
SN - 0385-8146
IS - 2
ER -