Salicylate-induced morphological changes of isolated inner hair cells and outer hair cells from guinea-pig cochlea

Takashi Kimitsuki, Yasuhiro Kakazu, Nozomu Matsumoto, teppei noda, Noritaka Komune, Shizuo Komune

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: The aim of this study is to investigate the salicylate-induced morphological changes of cochlear inner hair cells (IHCs) and outer hair cells (OHCs). Methods: IHCs and OHCs were acutely isolated from the guinea-pig cochlea. Cells were observed under the inverted microscope and 10 mmol/L sodium salicylate solutions or 0.01 mmol/L dexamethasone-plus-salicylate solutions were applied. The cell length or the ratio between the length and width was the indices of the morphological changes in cells. Results: Isolated IHCs did not demonstrate any significant changes in sodium salicylate solutions in 20 min and in 40 min, whereas OHCs were shortened by the 10 mmol/L sodium salicylate to 83% in 20 min and 75% in 40 min. There were no significant differences between in the dexamethasone-plus-salicylate solutions and in the control solutions after 20 min and 40 min both in IHCs and OHCs. Conclusions: Although salicylate affected the isolated OHCs from guinea-pig cochlea, IHCs were not changed morphologically by sodium salicylate applications. Dexamethasone inhibited the salicylate-induced morphological changes of OHCs.

Original languageEnglish
Pages (from-to)152-156
Number of pages5
JournalAuris Nasus Larynx
Volume36
Issue number2
DOIs
Publication statusPublished - Apr 1 2009
Externally publishedYes

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Outer Auditory Hair Cells
Inner Auditory Hair Cells
Salicylates
Cochlea
Sodium Salicylate
Guinea Pigs
Dexamethasone

All Science Journal Classification (ASJC) codes

  • Surgery
  • Otorhinolaryngology

Cite this

Salicylate-induced morphological changes of isolated inner hair cells and outer hair cells from guinea-pig cochlea. / Kimitsuki, Takashi; Kakazu, Yasuhiro; Matsumoto, Nozomu; noda, teppei; Komune, Noritaka; Komune, Shizuo.

In: Auris Nasus Larynx, Vol. 36, No. 2, 01.04.2009, p. 152-156.

Research output: Contribution to journalArticle

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