Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Narissara Jariyapan, Wej Choochote, Atchariya Jitpakdi, Thasaneeya Harnnoi, Padet Siriyasatein, Mark C Wilkinson, Anuluck Junkum, Paul A Bates

Research output: Contribution to journalArticle

Abstract

Salivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3--10 days was approximately 1.08 +/- 0.04 microg/female and 0.1 +/- 0.05 microg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.

Original languageEnglish
Pages (from-to)5-10
Number of pages6
JournalRevista do Instituto de Medicina Tropical de Sao Paulo
Volume49
Issue number1
Publication statusPublished - Mar 27 2007

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Salivary Proteins and Peptides
Anopheles
Culicidae
Diptera
Malaria
Salivary Glands
Apyrase
Proteins
alpha-Glucosidases
Isoelectric Point
Blood Glucose
Blood Proteins
Polyacrylamide Gel Electrophoresis
Enzymes

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Jariyapan, N., Choochote, W., Jitpakdi, A., Harnnoi, T., Siriyasatein, P., Wilkinson, M. C., ... Bates, P. A. (2007). Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae). Revista do Instituto de Medicina Tropical de Sao Paulo, 49(1), 5-10.

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera : Culicidae). / Jariyapan, Narissara; Choochote, Wej; Jitpakdi, Atchariya; Harnnoi, Thasaneeya; Siriyasatein, Padet; Wilkinson, Mark C; Junkum, Anuluck; Bates, Paul A.

In: Revista do Instituto de Medicina Tropical de Sao Paulo, Vol. 49, No. 1, 27.03.2007, p. 5-10.

Research output: Contribution to journalArticle

Jariyapan, N, Choochote, W, Jitpakdi, A, Harnnoi, T, Siriyasatein, P, Wilkinson, MC, Junkum, A & Bates, PA 2007, 'Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)', Revista do Instituto de Medicina Tropical de Sao Paulo, vol. 49, no. 1, pp. 5-10.
Jariyapan, Narissara ; Choochote, Wej ; Jitpakdi, Atchariya ; Harnnoi, Thasaneeya ; Siriyasatein, Padet ; Wilkinson, Mark C ; Junkum, Anuluck ; Bates, Paul A. / Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera : Culicidae). In: Revista do Instituto de Medicina Tropical de Sao Paulo. 2007 ; Vol. 49, No. 1. pp. 5-10.
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