Satellite cell activation in stretched skeletal muscle and the role of nitric oxide and hepatocyte growth factor

Ryuichi Tatsumi, Xiaosong Liu, Antonio Pulido, Mark Morales, Tomowa Sakata, Sharon Dial, Akihito Hattori, Yoshihide Ikeuchi, Ronald E. Allen

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Abstract

In the present study, we examined the roles of hepatocyte growth factor (HGF) and nitric oxide (NO) in the activation of satellite cells in passively stretched rat skeletal muscle. A hindlimb suspension model was developed in which the vastus, adductor, and gracilis muscles were subjected to stretch for 1 h. Satellite cells were activated by stretch determined on the basis of 5-bromo-2′-deoxyuridine (BrdU) incorporation in vivo. Extracts from stretched muscles stimulated BrdU incorporation in freshly isolated control rat satellite cells in a concentration-dependent manner. Extracts from stretched muscles contained the active form of HGF, and the satellite cell-activating activity could be neutralized by incubation with anti-HGF antibody. The involvement of NO was investigated by administering nitro-L-arginine methyl ester (L-NAME) or the inactive enantiomer NG-nitro-D-arginine methyl ester HCl (D-NAME) before stretch treatment. In vivo activation of satellite cells in stretched muscle was not inhibited by D-NAME but was inhibited by L-NAME. The activity of stretched muscle extract was abolished by L-NAME treatment but could be restored by the addition of HGF, indicating that the extract was not inhibitory. Finally, NO synthase activity in stretched and unstretched muscles was assayed in muscle extracts immediately after 2-h stretch treatment and was found to be elevated in stretched muscle but not in stretched muscle from L-NAME-treated rats. The results of these experiments demonstrate that stretching muscle liberates HGF in a NO-dependent manner, which can activate satellite cells.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume290
Issue number6
DOIs
Publication statusPublished - Jun 1 2006

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Hepatocyte Growth Factor
Nitric Oxide
Skeletal Muscle
Muscles
NG-Nitroarginine Methyl Ester
Bromodeoxyuridine
Hindlimb Suspension
Nitric Oxide Synthase
Therapeutics
Antibodies

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

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Satellite cell activation in stretched skeletal muscle and the role of nitric oxide and hepatocyte growth factor. / Tatsumi, Ryuichi; Liu, Xiaosong; Pulido, Antonio; Morales, Mark; Sakata, Tomowa; Dial, Sharon; Hattori, Akihito; Ikeuchi, Yoshihide; Allen, Ronald E.

In: American Journal of Physiology - Cell Physiology, Vol. 290, No. 6, 01.06.2006.

Research output: Contribution to journalArticle

Tatsumi, Ryuichi ; Liu, Xiaosong ; Pulido, Antonio ; Morales, Mark ; Sakata, Tomowa ; Dial, Sharon ; Hattori, Akihito ; Ikeuchi, Yoshihide ; Allen, Ronald E. / Satellite cell activation in stretched skeletal muscle and the role of nitric oxide and hepatocyte growth factor. In: American Journal of Physiology - Cell Physiology. 2006 ; Vol. 290, No. 6.
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