Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury

Yusuke Sato, Mai Khoi Q. Do, Takahiro Suzuki, Hideaki Ohtsubo, Wataru Mizunoya, Mako Nakamura, Mitsuhiro Furuse, Yoshihide Ikeuchi, Ryuichi Tatsumi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed a heretofore unexplored role of satellite cells as a key source of a secreted neural chemorepellent semaphorin 3A (Sema3A) expression. In order to verify this concept, there is still a critical need to provide direct evidence to show the up-regulation of Sema3A protein in satellite cells in vivo upon muscle injury. The present study employed a Sema3A/MyoD double-immunohistochemical staining for cryo-sections prepared from cardiotoxin injected gastrocnemius muscle of adult mouse lower hind-limb. Results clearly demonstrated that Sema3A expression was up-regulated in myogenic differentiation-positive satellite cells at 4-12 days post-injury period, the time that corresponds to the cell differentiation phase characterized by increasing myogenin messenger RNA expression. This direct proof encourages a possible implication of satellite cells in the spatiotemporal regulation of extracellular Sema3A concentrations, which potentially ensures coordinating a delay in neurite sprouting and re-attachment of motoneuron terminals onto damaged muscle fibers early in muscle regeneration in synchrony with recovery of muscle-fiber integrity.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalAnimal Science Journal
Volume84
Issue number2
DOIs
Publication statusPublished - Feb 1 2013

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Semaphorin-3A
Muscles
muscles
Wounds and Injuries
muscle fibers
cells
Cardiotoxins
neurites
muscle development
Myogenin
innervation
motor neurons
messenger RNA
sprouting
limbs (animal)
axons
cell differentiation
Motor Neurons
Neurites
Regeneration

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury. / Sato, Yusuke; Do, Mai Khoi Q.; Suzuki, Takahiro; Ohtsubo, Hideaki; Mizunoya, Wataru; Nakamura, Mako; Furuse, Mitsuhiro; Ikeuchi, Yoshihide; Tatsumi, Ryuichi.

In: Animal Science Journal, Vol. 84, No. 2, 01.02.2013, p. 185-189.

Research output: Contribution to journalArticle

Sato, Yusuke ; Do, Mai Khoi Q. ; Suzuki, Takahiro ; Ohtsubo, Hideaki ; Mizunoya, Wataru ; Nakamura, Mako ; Furuse, Mitsuhiro ; Ikeuchi, Yoshihide ; Tatsumi, Ryuichi. / Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury. In: Animal Science Journal. 2013 ; Vol. 84, No. 2. pp. 185-189.
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