Scale up of cerrena unicolor lacease production

Beata Rola, Maciej Karaśkiewicz, Dominika Majdecka, Iwona Mazur, Renata Bilewicz, Jerzy Rogalski, Shoji Ohga

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of different carbon source in culture medium of wood-degrading basidiomycete Cerrena unicolor C-139 were investigated. The maximal growth and lacease synthesis in shaken flask was observed in mineral salts broth containing maltose as the carbon source and asparagine as the nitrogen source (C/N= 17.45). The maximal lacease activities in this condition (in the end of fungus expotential growth phase) was 4-times higher (28000 nkat/l) according to control conditions with glucose. The comparison of different inoculum age preparations for laboratory scale fermentor seeding display that the best results (154 078 nkat/l) were observed when the mycelium comes from the early expotential growth phase. In the scale-up process in SIP type 401 fermentor controlled automatically by the cascade of agitation (200-500 rpm) and gas flow of air (2-3 SLMP) giving the dissolved oxygen concentration in the range of 35-100% of initial amount, (enriched from 2 to 6 day of cultivation by the sequential addition of 10μM cupric ions) the highest lacease activity in the medium with glucose (150,000 nkat/l) or maltose (145,000 nkat/l) at 8-th and 10-th day of cultivation were observed. The after culture fluids from these time were next concentrated and partially purified giving the crude lacease preparations Lac-G and Lac-M (from media with glucose or maltose respectively). The obtained preparations compared by ion-exchange HPLC, electrophorese and cyclic voltammetry gave no significant differences in their properties.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume58
Issue number2
Publication statusPublished - Sep 1 2013

Fingerprint

Maltose
maltose
fermenters
Bioreactors
Glucose
glucose
Carbon
Growth
Basidiomycota
carbon
Ion Exchange
Asparagine
Mycelium
agitation
ion exchange
asparagine
air flow
dissolved oxygen
mycelium
Minerals

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Rola, B., Karaśkiewicz, M., Majdecka, D., Mazur, I., Bilewicz, R., Rogalski, J., & Ohga, S. (2013). Scale up of cerrena unicolor lacease production. Journal of the Faculty of Agriculture, Kyushu University, 58(2), 231-238.

Scale up of cerrena unicolor lacease production. / Rola, Beata; Karaśkiewicz, Maciej; Majdecka, Dominika; Mazur, Iwona; Bilewicz, Renata; Rogalski, Jerzy; Ohga, Shoji.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 58, No. 2, 01.09.2013, p. 231-238.

Research output: Contribution to journalArticle

Rola, B, Karaśkiewicz, M, Majdecka, D, Mazur, I, Bilewicz, R, Rogalski, J & Ohga, S 2013, 'Scale up of cerrena unicolor lacease production', Journal of the Faculty of Agriculture, Kyushu University, vol. 58, no. 2, pp. 231-238.
Rola B, Karaśkiewicz M, Majdecka D, Mazur I, Bilewicz R, Rogalski J et al. Scale up of cerrena unicolor lacease production. Journal of the Faculty of Agriculture, Kyushu University. 2013 Sep 1;58(2):231-238.
Rola, Beata ; Karaśkiewicz, Maciej ; Majdecka, Dominika ; Mazur, Iwona ; Bilewicz, Renata ; Rogalski, Jerzy ; Ohga, Shoji. / Scale up of cerrena unicolor lacease production. In: Journal of the Faculty of Agriculture, Kyushu University. 2013 ; Vol. 58, No. 2. pp. 231-238.
@article{957fe531b4e142a0b7b8c8fbe450298a,
title = "Scale up of cerrena unicolor lacease production",
abstract = "The effect of different carbon source in culture medium of wood-degrading basidiomycete Cerrena unicolor C-139 were investigated. The maximal growth and lacease synthesis in shaken flask was observed in mineral salts broth containing maltose as the carbon source and asparagine as the nitrogen source (C/N= 17.45). The maximal lacease activities in this condition (in the end of fungus expotential growth phase) was 4-times higher (28000 nkat/l) according to control conditions with glucose. The comparison of different inoculum age preparations for laboratory scale fermentor seeding display that the best results (154 078 nkat/l) were observed when the mycelium comes from the early expotential growth phase. In the scale-up process in SIP type 401 fermentor controlled automatically by the cascade of agitation (200-500 rpm) and gas flow of air (2-3 SLMP) giving the dissolved oxygen concentration in the range of 35-100{\%} of initial amount, (enriched from 2 to 6 day of cultivation by the sequential addition of 10μM cupric ions) the highest lacease activity in the medium with glucose (150,000 nkat/l) or maltose (145,000 nkat/l) at 8-th and 10-th day of cultivation were observed. The after culture fluids from these time were next concentrated and partially purified giving the crude lacease preparations Lac-G and Lac-M (from media with glucose or maltose respectively). The obtained preparations compared by ion-exchange HPLC, electrophorese and cyclic voltammetry gave no significant differences in their properties.",
author = "Beata Rola and Maciej Karaśkiewicz and Dominika Majdecka and Iwona Mazur and Renata Bilewicz and Jerzy Rogalski and Shoji Ohga",
year = "2013",
month = "9",
day = "1",
language = "English",
volume = "58",
pages = "231--238",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "2",

}

TY - JOUR

T1 - Scale up of cerrena unicolor lacease production

AU - Rola, Beata

AU - Karaśkiewicz, Maciej

AU - Majdecka, Dominika

AU - Mazur, Iwona

AU - Bilewicz, Renata

AU - Rogalski, Jerzy

AU - Ohga, Shoji

PY - 2013/9/1

Y1 - 2013/9/1

N2 - The effect of different carbon source in culture medium of wood-degrading basidiomycete Cerrena unicolor C-139 were investigated. The maximal growth and lacease synthesis in shaken flask was observed in mineral salts broth containing maltose as the carbon source and asparagine as the nitrogen source (C/N= 17.45). The maximal lacease activities in this condition (in the end of fungus expotential growth phase) was 4-times higher (28000 nkat/l) according to control conditions with glucose. The comparison of different inoculum age preparations for laboratory scale fermentor seeding display that the best results (154 078 nkat/l) were observed when the mycelium comes from the early expotential growth phase. In the scale-up process in SIP type 401 fermentor controlled automatically by the cascade of agitation (200-500 rpm) and gas flow of air (2-3 SLMP) giving the dissolved oxygen concentration in the range of 35-100% of initial amount, (enriched from 2 to 6 day of cultivation by the sequential addition of 10μM cupric ions) the highest lacease activity in the medium with glucose (150,000 nkat/l) or maltose (145,000 nkat/l) at 8-th and 10-th day of cultivation were observed. The after culture fluids from these time were next concentrated and partially purified giving the crude lacease preparations Lac-G and Lac-M (from media with glucose or maltose respectively). The obtained preparations compared by ion-exchange HPLC, electrophorese and cyclic voltammetry gave no significant differences in their properties.

AB - The effect of different carbon source in culture medium of wood-degrading basidiomycete Cerrena unicolor C-139 were investigated. The maximal growth and lacease synthesis in shaken flask was observed in mineral salts broth containing maltose as the carbon source and asparagine as the nitrogen source (C/N= 17.45). The maximal lacease activities in this condition (in the end of fungus expotential growth phase) was 4-times higher (28000 nkat/l) according to control conditions with glucose. The comparison of different inoculum age preparations for laboratory scale fermentor seeding display that the best results (154 078 nkat/l) were observed when the mycelium comes from the early expotential growth phase. In the scale-up process in SIP type 401 fermentor controlled automatically by the cascade of agitation (200-500 rpm) and gas flow of air (2-3 SLMP) giving the dissolved oxygen concentration in the range of 35-100% of initial amount, (enriched from 2 to 6 day of cultivation by the sequential addition of 10μM cupric ions) the highest lacease activity in the medium with glucose (150,000 nkat/l) or maltose (145,000 nkat/l) at 8-th and 10-th day of cultivation were observed. The after culture fluids from these time were next concentrated and partially purified giving the crude lacease preparations Lac-G and Lac-M (from media with glucose or maltose respectively). The obtained preparations compared by ion-exchange HPLC, electrophorese and cyclic voltammetry gave no significant differences in their properties.

UR - http://www.scopus.com/inward/record.url?scp=84885766969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885766969&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84885766969

VL - 58

SP - 231

EP - 238

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 2

ER -