Scavenger receptor for lipoteichoic acid is involved in the potent ability of Lactobacillus plantarum strain L-137 to stimulate production of interleukin-12p40

Shinya Hatano, Yoshitaka Hirose, Yoshihiro Yamamoto, Shinji Murosaki, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

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    Abstract

    Heat-killed Lactobacillus plantarum strain L-137 (HK L-137) is a more potent inducer of interleukin (IL)-12 than other heat-killed Lactobacillus strains. To elucidate the mechanism involved in this IL-12p40 induction, we compared HK L-137 with heat-killed L. plantarum strain JCM1149 (HK JCM1149) by nuclear magnetic resonance and mass spectrometry. Results showed that HK L-137 contained lipoteichoic acid (LTA) with a chemical structure similar to that of JCM1149, except for a lower degree of glucosyl substitution in the poly(glycerol phosphate) backbone. Lysozyme sensitivity and electrophoretic moiety analysis revealed that HK L-137 exposed more LTA on its cell surface than HK JCM1149. Phagocytosis of HK L-137 by splenic adherent cells was significantly greater than that of HK JCM1149. Anti-LTA antibody and anti-scavenger receptor-A (SR-A) antibody selectively inhibited phagocytosis of HK L-137, as well as IL-12p40 production, by splenic adherent cells. Thus, a higher efficiency of phagocytosis of HK L-137 via SR-A for LTA is responsible for the potent IL-12p40 induction.

    Original languageEnglish
    Pages (from-to)321-331
    Number of pages11
    JournalInternational Immunopharmacology
    Volume25
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 2015

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    1-phenyl-6,7-dihydroxy-isochroman
    Scavenger Receptors
    Lactobacillus plantarum
    Interleukins
    Phagocytosis
    Hot Temperature
    lipoteichoic acid
    Lactobacillus
    Interleukin-12
    Muramidase
    Anti-Idiotypic Antibodies
    Mass Spectrometry
    Magnetic Resonance Spectroscopy

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology
    • Pharmacology

    Cite this

    Scavenger receptor for lipoteichoic acid is involved in the potent ability of Lactobacillus plantarum strain L-137 to stimulate production of interleukin-12p40. / Hatano, Shinya; Hirose, Yoshitaka; Yamamoto, Yoshihiro; Murosaki, Shinji; Yoshikai, Yasunobu.

    In: International Immunopharmacology, Vol. 25, No. 2, 01.01.2015, p. 321-331.

    Research output: Contribution to journalArticle

    Hatano, Shinya ; Hirose, Yoshitaka ; Yamamoto, Yoshihiro ; Murosaki, Shinji ; Yoshikai, Yasunobu. / Scavenger receptor for lipoteichoic acid is involved in the potent ability of Lactobacillus plantarum strain L-137 to stimulate production of interleukin-12p40. In: International Immunopharmacology. 2015 ; Vol. 25, No. 2. pp. 321-331.
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