Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier

Teruaki Hasegawa, Tomohisa Fujisawa, Munenori Numata, Takahiro Matsumoto, Mariko Umeda, Ryouji Karinaga, Masami Mizu, Kazuya Koumoto, Taro Kimura, Shiro Okumura, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Schizophyllans carrying β-lactoside and α-mannoside appendages were prepared from native schizophyllan through NaIO4 oxidation followed by reductive amination using aminoethyl-β-lactoside and α-mannoside, respectively. The resulting schizophyllans form stable macromolecular complexes with polynucleotides, such as poly(C) and poly(dA). Specific affinity between these macromolecular complexes and saccharide-binding proteins was demonstrated by surface plasmon resonance and agarose gel staining assays. β-Lactoside-appended schizophyllan enhanced an antisense activity in hepatocytes which express lactoside-binding proteins on their cell surfaces.

Original languageEnglish
Pages (from-to)3091-3098
Number of pages8
JournalOrganic and Biomolecular Chemistry
Volume2
Issue number21
DOIs
Publication statusPublished - Nov 7 2004

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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