Schizosaccharomyces pombe UDP-galactose transporter

Identification of its functional form through cDNA cloning and expression in mammalian cells

Hiroaki Segawa, Nobuhiro Ishida, Kaoru Takegawa, Masao Kawakita

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The Schizosaccharomyces pombe UDP-galactose transporter cDNA (SpUGT cDNA), encoding the product of the gms1+ gene which consists of two exon sequences separated by a 173-bp intron, was cloned by RT-PCR. Its product, a hydrophobic protein of 353 amino acid residues resembling its human counterpart, was expressed in the Golgi membranes of UDP-galactose transporter-deficient Lec8 cells, and complemented the genetic defect of the mutant cells. This indicated that SpUGT cDNA encodes the functional S. pombe UDP-galactose transporter. The product of an ORF found in the second exon, which was previously assumed to be the S. pombe UDP-galactose transporter, thus represents an inactive, truncated form of the SpUGT protein. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)295-298
Number of pages4
JournalFEBS Letters
Volume451
Issue number3
DOIs
Publication statusPublished - May 28 1999

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Schizosaccharomyces
Cloning
Organism Cloning
Complementary DNA
Cells
Exons
Introns
Open Reading Frames
Proteins
Genes
UDP-galactose translocator
Membranes
Amino Acids
Polymerase Chain Reaction
Defects

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Schizosaccharomyces pombe UDP-galactose transporter : Identification of its functional form through cDNA cloning and expression in mammalian cells. / Segawa, Hiroaki; Ishida, Nobuhiro; Takegawa, Kaoru; Kawakita, Masao.

In: FEBS Letters, Vol. 451, No. 3, 28.05.1999, p. 295-298.

Research output: Contribution to journalArticle

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