TY - JOUR
T1 - Screening and confirmatory methods for the analysis of macrocyclic lactone mycotoxins by CE with amperometric detection
AU - Arribas, Alberto Sánchez
AU - Bermejo, Esperanza
AU - Zapardiel, Antonio
AU - Tellez Lozano, Helena
AU - Rodríguez-Flores, Juana
AU - Zougagh, Mohammed
AU - Ríos, Ángel
AU - Chicharro, Manuel
PY - 2009/4/27
Y1 - 2009/4/27
N2 - A simple analytical scheme for the screening and quantification of zearalenone and its metabolites, α-zearalenol and β-zearalenol, is reported. Extracts from maize flour samples were collected by supercritical fluid extraction and afterwards, they were analyzed by CE with amperometric detection. This scheme allowed a rapid and reliable identification of contaminated flour samples according to the reference value established for zearalenone by directive 2005/38/EC (200 μg/kg). The sample screening method was carried out by CZE using 25 mM borate separation buffer at pH 9.2 and 25.0 kV as separation voltage, monitoring the amperometric signal at +700 mV with a carbon paste electrode. In this way, total amount of mycotoxins was determined and samples were processed in 4 min with a detection limit of 12 μg/L, enough to discriminate between positive (more than 200 μg/L total mycotoxins) and negative samples (less than 200 μg/L total mycotoxins). Positive samples were then subjected to CZE separation and quantification of each analyte was done with 50 mM borate running buffer modified with 30% methanol at pH 9.7 and 17.5 kV as separation voltage. Under these conditions, separation was achieved in 15 min with detection limits from 20 to 35 μg/L for each analyte.
AB - A simple analytical scheme for the screening and quantification of zearalenone and its metabolites, α-zearalenol and β-zearalenol, is reported. Extracts from maize flour samples were collected by supercritical fluid extraction and afterwards, they were analyzed by CE with amperometric detection. This scheme allowed a rapid and reliable identification of contaminated flour samples according to the reference value established for zearalenone by directive 2005/38/EC (200 μg/kg). The sample screening method was carried out by CZE using 25 mM borate separation buffer at pH 9.2 and 25.0 kV as separation voltage, monitoring the amperometric signal at +700 mV with a carbon paste electrode. In this way, total amount of mycotoxins was determined and samples were processed in 4 min with a detection limit of 12 μg/L, enough to discriminate between positive (more than 200 μg/L total mycotoxins) and negative samples (less than 200 μg/L total mycotoxins). Positive samples were then subjected to CZE separation and quantification of each analyte was done with 50 mM borate running buffer modified with 30% methanol at pH 9.7 and 17.5 kV as separation voltage. Under these conditions, separation was achieved in 15 min with detection limits from 20 to 35 μg/L for each analyte.
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U2 - 10.1002/elps.200800305
DO - 10.1002/elps.200800305
M3 - Article
C2 - 19156758
AN - SCOPUS:64049117017
VL - 30
SP - 499
EP - 506
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 3
ER -