Screening of arylboronic acids to search for a strong inhibitor for γ-glutamyl transpeptidase (γ-GTP)

Hikaru Suenaga, Kazuaki Nakashima, Masafumi Mikami, Hiromasa Yamamoto, Tony D. James, K. R.A. Samankumara Sandanayake, Seiji Shinkai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In a study of the development of a strong inhibitor for γ-glutamyl transpeptidase (γ-GTP), the inhibitory effect of 21 arylboronic acids was determined in the γ-GTP-mediated hydrolysis of N-(L-γ-glutamyl)-4-nitroanilide. It was shown that most boronic acids can moderately inhibit the enzyme activity and, in particular, the inhibitory effects of (3-formylphenyl)boronic acid and (3-carboxyphenyl)boronic acid (9% and 12%, respectively) are comparable to that reported for the binary L-serine-plus-borate inhibition system (7%). Furthermore, it was shown that the addition of physiologically nontoxic saccharides is very effective in regenerating the enzyme activity. This effect is attributed to the competitive binding of saccharides with the boronic acid inhibitor to pull it out into the water phase. Thus, this paper offers a novel methodology for inhibition and control of γ-GTP activity.

Original languageEnglish
Pages (from-to)44-48
Number of pages5
JournalRecueil des Travaux Chimiques des Pays-Bas-Journal of the Royal Netherlands
Volume115
Issue number1
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Boronic Acids
gamma-Glutamyltransferase
Screening
Acids
Enzyme activity
Guanosine Triphosphate
Borates
Serine
Hydrolysis
Water

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Suenaga, H., Nakashima, K., Mikami, M., Yamamoto, H., James, T. D., Samankumara Sandanayake, K. R. A., & Shinkai, S. (1996). Screening of arylboronic acids to search for a strong inhibitor for γ-glutamyl transpeptidase (γ-GTP). Recueil des Travaux Chimiques des Pays-Bas-Journal of the Royal Netherlands, 115(1), 44-48.

Screening of arylboronic acids to search for a strong inhibitor for γ-glutamyl transpeptidase (γ-GTP). / Suenaga, Hikaru; Nakashima, Kazuaki; Mikami, Masafumi; Yamamoto, Hiromasa; James, Tony D.; Samankumara Sandanayake, K. R.A.; Shinkai, Seiji.

In: Recueil des Travaux Chimiques des Pays-Bas-Journal of the Royal Netherlands, Vol. 115, No. 1, 01.01.1996, p. 44-48.

Research output: Contribution to journalArticle

Suenaga, H, Nakashima, K, Mikami, M, Yamamoto, H, James, TD, Samankumara Sandanayake, KRA & Shinkai, S 1996, 'Screening of arylboronic acids to search for a strong inhibitor for γ-glutamyl transpeptidase (γ-GTP)', Recueil des Travaux Chimiques des Pays-Bas-Journal of the Royal Netherlands, vol. 115, no. 1, pp. 44-48.
Suenaga H, Nakashima K, Mikami M, Yamamoto H, James TD, Samankumara Sandanayake KRA et al. Screening of arylboronic acids to search for a strong inhibitor for γ-glutamyl transpeptidase (γ-GTP). Recueil des Travaux Chimiques des Pays-Bas-Journal of the Royal Netherlands. 1996 Jan 1;115(1):44-48.
Suenaga, Hikaru ; Nakashima, Kazuaki ; Mikami, Masafumi ; Yamamoto, Hiromasa ; James, Tony D. ; Samankumara Sandanayake, K. R.A. ; Shinkai, Seiji. / Screening of arylboronic acids to search for a strong inhibitor for γ-glutamyl transpeptidase (γ-GTP). In: Recueil des Travaux Chimiques des Pays-Bas-Journal of the Royal Netherlands. 1996 ; Vol. 115, No. 1. pp. 44-48.
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