Screening of potential molecular targets for colorectal cancer therapy

Kimi Honma, Ichiro Takemasa, Ryo Matoba, Yusuke Yamamoto, Fumitaka Takeshita, Masaki Mori, Morito Monden, Kenichi Matsubara, Takahiro Ochiya

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11 Citations (Scopus)

Abstract

Colorectal cancer is a leading cause of cancer death worldwide. To identify molecular targets for colorectal cancer therapy, we tested small interfering RNAs (siRNAs) against 97 genes whose expression was elevated in human colorectal cancer tissues for the ability to promote apoptosis of human colorectal cancer cells (HT-29 cells). The results indicate that the downregulation of PSMA7 (proteasome subunit, α-type, 7) and RAN (ras-related nuclear protein) most efficiently induced apoptosis of HT-29 cells. PSMA7 and RAN were highly expressed in colorectal cancer cell lines compared with normal colon tissues. Furthermore, PSMA7 and RAN were overexpressed in not only colon tumor tissues but also the other tumor tissues. Moreover, in vivo delivery of PSMA7 siRNA and RAN siRNA markedly induced apoptosis in HT-29 xenograft tumors in mice. Thus, silencing of PSMA7 and RAN induces cancer cells to undergo apoptosis, and PSMA7 and RAN might be promising new molecular targets for drug and RNA interference-based therapeutics against colorectal cancer.

Original languageEnglish
Pages (from-to)243-257
Number of pages15
JournalInternational Journal of General Medicine
Volume2
Publication statusPublished - Dec 1 2009
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Honma, K., Takemasa, I., Matoba, R., Yamamoto, Y., Takeshita, F., Mori, M., ... Ochiya, T. (2009). Screening of potential molecular targets for colorectal cancer therapy. International Journal of General Medicine, 2, 243-257.