Search for proteins required for accurate gene expression under oxidative stress: Roles of Guanylate Kinase and RNA Polymerase

Hachiro Inokuchi, Riyoko Ito, Takeshi Sekiguchi, Mutsuo Sekiguchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In aerobically growing cells, in which reactive oxygen species are produced, the guanine base is oxidized to 8-oxo-7,8-dihydroguanine, which can pair with adenine as well as cytosine. This mispairing causes alterations in gene expression, and cells possess mechanisms to prevent such outcomes. In Escherichia coli, 8-oxo-7,8-dihydroguanine-related phenotypic suppression of lacZ amber is enhanced by mutations in genes related to the prevention of abnormal protein synthesis under oxidative stress. A genome-wide search for the genes responsible, followed by DNA sequence determination, revealed that specific amino acid changes in guanylate kinase and in the β and β′ subunits ofRNApolymerase cause elevated levels of phenotypic suppression, specifically under aerobic conditions. The involvement of the DnaB, DnaN, and MsbA proteins, which are involved in DNA replication and in preserving the membrane structure, was also noted. Interactions of these proteins with each other and also with other molecules may be important for preventing errors in gene expression.

Original languageEnglish
Pages (from-to)32952-32962
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number46
DOIs
Publication statusPublished - Nov 15 2013

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this