Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish, Carassius auratus

Nobuo Suzuki, Masayuki Sato, Hossam F. Nassar, Fagr Kh Abdel-Gawad, Samah M. Bassem, Koji Yachiguchi, Yoshiaki Tabuchi, Masato Endo, Toshio Sekiguchi, Makoto Urata, Atsuhiko Hattori, Hiroyuki Mishima, Youhei Shimasaki, Yuji Oshima, Chun Sang Hong, Fumiya Makino, Ning Tang, Akira Toriba, Kazuichi Hayakawa

Research output: Contribution to journalArticle

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Abstract

We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from two sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We were able to detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added to culture medium at dilution rates of 50, 100, and 500, and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, but TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-κB ligand) decreased significantly, as did the ALP enzyme activity. In fact, ALP activity decreased on treatment with PAHs and NPAHs. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influences bone metabolism in teleosts.

Original languageEnglish
Pages (from-to)407-413
Number of pages7
JournalZoological science
Volume33
Issue number4
DOIs
Publication statusPublished - Aug 1 2016

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Carassius auratus
goldfish
polycyclic aromatic hydrocarbons
seawater
alkaline phosphatase
acid phosphatase
osteoclasts
osteoblasts
bioassays
bone metabolism
osteocalcin
Red Sea
Mediterranean Sea
sampling
culture media
bones
enzyme activity
receptors

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish, Carassius auratus. / Suzuki, Nobuo; Sato, Masayuki; Nassar, Hossam F.; Abdel-Gawad, Fagr Kh; Bassem, Samah M.; Yachiguchi, Koji; Tabuchi, Yoshiaki; Endo, Masato; Sekiguchi, Toshio; Urata, Makoto; Hattori, Atsuhiko; Mishima, Hiroyuki; Shimasaki, Youhei; Oshima, Yuji; Hong, Chun Sang; Makino, Fumiya; Tang, Ning; Toriba, Akira; Hayakawa, Kazuichi.

In: Zoological science, Vol. 33, No. 4, 01.08.2016, p. 407-413.

Research output: Contribution to journalArticle

Suzuki, N, Sato, M, Nassar, HF, Abdel-Gawad, FK, Bassem, SM, Yachiguchi, K, Tabuchi, Y, Endo, M, Sekiguchi, T, Urata, M, Hattori, A, Mishima, H, Shimasaki, Y, Oshima, Y, Hong, CS, Makino, F, Tang, N, Toriba, A & Hayakawa, K 2016, 'Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish, Carassius auratus', Zoological science, vol. 33, no. 4, pp. 407-413. https://doi.org/10.2108/zs150211
Suzuki, Nobuo ; Sato, Masayuki ; Nassar, Hossam F. ; Abdel-Gawad, Fagr Kh ; Bassem, Samah M. ; Yachiguchi, Koji ; Tabuchi, Yoshiaki ; Endo, Masato ; Sekiguchi, Toshio ; Urata, Makoto ; Hattori, Atsuhiko ; Mishima, Hiroyuki ; Shimasaki, Youhei ; Oshima, Yuji ; Hong, Chun Sang ; Makino, Fumiya ; Tang, Ning ; Toriba, Akira ; Hayakawa, Kazuichi. / Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish, Carassius auratus. In: Zoological science. 2016 ; Vol. 33, No. 4. pp. 407-413.
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abstract = "We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from two sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We were able to detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added to culture medium at dilution rates of 50, 100, and 500, and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, but TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-κB ligand) decreased significantly, as did the ALP enzyme activity. In fact, ALP activity decreased on treatment with PAHs and NPAHs. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influences bone metabolism in teleosts.",
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AU - Nassar, Hossam F.

AU - Abdel-Gawad, Fagr Kh

AU - Bassem, Samah M.

AU - Yachiguchi, Koji

AU - Tabuchi, Yoshiaki

AU - Endo, Masato

AU - Sekiguchi, Toshio

AU - Urata, Makoto

AU - Hattori, Atsuhiko

AU - Mishima, Hiroyuki

AU - Shimasaki, Youhei

AU - Oshima, Yuji

AU - Hong, Chun Sang

AU - Makino, Fumiya

AU - Tang, Ning

AU - Toriba, Akira

AU - Hayakawa, Kazuichi

PY - 2016/8/1

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