Secondary structural features of modules M2 and M3 of barnase in solution by NMR experiment and distance geometry calculation

Teikichi Ikura, Nobuhiro Gō, Daisuke Kohda, Fuyuhiko Inagaki, Hiroshi Yanagawa, Masuyo Kawabata, Shun-Ichiro Kawabata, Sadaaki Iwanaga, Tosiyuki Noguti, Mitiko Gō

Research output: Contribution to journalArticle

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Abstract

Proteins consist of structural units such as globular domains, secondary structures, and modules. Modules were originally defined by partitioning a globular domain into compact regions, each of which is a contiguous polypeptide segment having a compact conformation. Since modules show close correlations with the intron positions of genes, they are regarded as primordial polypeptide pieces encoded by exons and shuffled, leading to yield new combination of them in early biological evolution. Do modules maintain their native conformations in solution when they are excised at their boundaries? In order to find answers to this question, we have synthesized modules of barnase, one of the bacterial RNases, and studied the solution structures of modules M2 (amino acid residues 24–52) and M3 (52–73) by 2D NMR studies. Some local secondary structures, α‐helix, and β‐turns in M2 and β‐turns in M3, were observed in the modules at the similar positions to those in the intact barnase but the overall state seems to be in a mixture of random and native conformations. The present result shows that the excised modules have propensity to form similar secondary structures to those of the intact barnase. © 1993 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)341-356
Number of pages16
JournalProteins: Structure, Function, and Bioinformatics
Volume16
Issue number4
DOIs
Publication statusPublished - Jan 1 1993

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Conformations
Nuclear magnetic resonance
Geometry
Biological Evolution
Peptides
Gene Order
Experiments
Ribonucleases
Introns
Exons
Genes
Amino Acids
Bacillus amyloliquefaciens ribonuclease
Proteins
Protein Structural Elements

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Secondary structural features of modules M2 and M3 of barnase in solution by NMR experiment and distance geometry calculation. / Ikura, Teikichi; Gō, Nobuhiro; Kohda, Daisuke; Inagaki, Fuyuhiko; Yanagawa, Hiroshi; Kawabata, Masuyo; Kawabata, Shun-Ichiro; Iwanaga, Sadaaki; Noguti, Tosiyuki; Gō, Mitiko.

In: Proteins: Structure, Function, and Bioinformatics, Vol. 16, No. 4, 01.01.1993, p. 341-356.

Research output: Contribution to journalArticle

Ikura, Teikichi ; Gō, Nobuhiro ; Kohda, Daisuke ; Inagaki, Fuyuhiko ; Yanagawa, Hiroshi ; Kawabata, Masuyo ; Kawabata, Shun-Ichiro ; Iwanaga, Sadaaki ; Noguti, Tosiyuki ; Gō, Mitiko. / Secondary structural features of modules M2 and M3 of barnase in solution by NMR experiment and distance geometry calculation. In: Proteins: Structure, Function, and Bioinformatics. 1993 ; Vol. 16, No. 4. pp. 341-356.
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