Secreted frizzled-related protein 1 promotes odontoblastic differentiation and reparative dentin formation in dental pulp cells

Keita Ipposhi, Atsushi Tomokiyo, Taiga Ono, Kozue Yamashita, Muhammad Anas Alhasan, Daigaku Hasegawa, Sayuri Hamano, Shinichiro Yoshida, Hideki Sugii, Tomohiro Itoyama, Marina Ogawa, Hidefumi Maeda

Research output: Contribution to journalArticlepeer-review

Abstract

Direct pulp capping is an effective treatment for preserving dental pulp against carious or traumatic pulp exposure via the formation of protective reparative dentin by odontoblast-like cells. Reparative dentin formation can be stimulated by several signaling molecules; therefore, we investigated the effects of secreted frizzled-related protein (SFRP) 1 that was reported to be strongly expressed in odontoblasts of newborn molar tooth germs on odontoblastic differentiation and reparative dentin formation. In developing rat incisors, cells in the dental pulp, cervical loop, and inner enamel epithelium, as well as ameloblasts and preodontoblasts, weakly expressed Sfrp1; however, Sfrp1 was strongly expressed in mature odontoblasts. Human dental pulp cells (hDPCs) showed stronger expression of SFRP1 compared with periodontal ligament cells and gingival cells. SFRP1 knockdown in hDPCs abolished calcium chloride-induced mineralized nodule formation and odontoblast-related gene expression and decreased BMP-2 gene expression. Conversely, SFRP1 stimulation enhanced nodule formation and expression of BMP-2. Direct pulp capping treatment with SFRP1 induced the formation of a considerable amount of reparative dentin that has a structure similar to primary dentin. Our results indicate that SFRP1 is crucial for dentinogenesis and is important in promoting reparative dentin formation in response to injury.

Original languageEnglish
Article number2491
JournalCells
Volume10
Issue number9
DOIs
Publication statusPublished - Sep 2021

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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