Selection of peptides with the recognition of stereoregular PMMA from phage library

Toshiki Sawada, Hisao Matsuno, Teruhiko Matsubara, Toshinori Sato, Tatsuki Kitayama, Takeshi Serizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To identify peptides with affinity selective for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), phage library displaying a heptamer random peptide was used. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. Binding affinity and specificity of these phage clones were quantitatively investigated by enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

Original languageEnglish
Title of host publication54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan
Pages2081
Number of pages1
Volume54
Edition1
Publication statusPublished - 2005
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period5/25/055/27/05

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Bacteriophages
Polymethyl methacrylates
Peptides
Assays
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Sawada, T., Matsuno, H., Matsubara, T., Sato, T., Kitayama, T., & Serizawa, T. (2005). Selection of peptides with the recognition of stereoregular PMMA from phage library. In 54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan (1 ed., Vol. 54, pp. 2081)

Selection of peptides with the recognition of stereoregular PMMA from phage library. / Sawada, Toshiki; Matsuno, Hisao; Matsubara, Teruhiko; Sato, Toshinori; Kitayama, Tatsuki; Serizawa, Takeshi.

54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2081.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sawada, T, Matsuno, H, Matsubara, T, Sato, T, Kitayama, T & Serizawa, T 2005, Selection of peptides with the recognition of stereoregular PMMA from phage library. in 54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan. 1 edn, vol. 54, pp. 2081, 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 5/25/05.
Sawada T, Matsuno H, Matsubara T, Sato T, Kitayama T, Serizawa T. Selection of peptides with the recognition of stereoregular PMMA from phage library. In 54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan. 1 ed. Vol. 54. 2005. p. 2081
Sawada, Toshiki ; Matsuno, Hisao ; Matsubara, Teruhiko ; Sato, Toshinori ; Kitayama, Tatsuki ; Serizawa, Takeshi. / Selection of peptides with the recognition of stereoregular PMMA from phage library. 54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan. Vol. 54 1. ed. 2005. pp. 2081
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