Selection of poly(lactide)s binding peptides from phage displayed peptide library

Hisao Matsuno, Jun Sekine, Hirohumi Yajima, Takeshi Seizawa

Research output: Contribution to conferencePaper

Abstract

Structurally well-defined synthetic polymers with stereoregularity, chirality, helicity, and assembly are versatile materials for conjugation with biomolecules, and hybridization of these polymers with various biomolecules such as polypeptide and protein will expand its potentialities for biomedical applications. In this paper, peptides that bind to poly(L-lactide) (PLLA) were selected from the 7-mer peptide library. The spin coated film of PLLA was formed on a glass plate, and the biopanning was carried out on the film with the phage display peptide library. After four rounds of biopanning, sixteen individual sequences were obtained from the 30 phage clones. We quantified the binding property between the selected peptides and PLLA by the enzyme linked immunosorbent assay (ELISA).

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 19 2006
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

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All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Matsuno, H., Sekine, J., Yajima, H., & Seizawa, T. (2006). Selection of poly(lactide)s binding peptides from phage displayed peptide library. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.