Selection of suitable reference genes for mRNA quantification studies using common marmoset tissues

Yoshinori Shimamoto, Hiroshi Kitamura, Kimie Niimi, Yasunaga Yoshikawa, Fumio Hoshi, Mayumi Ishizuka, Eiki Takahashi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The common marmoset (Callithrix jacchus) is increasingly being used as a non-human primate animal model in biomedical research. To perform accurate quantitative analysis of gene expression by quantitative reverse transcription polymerase chain reaction, reliable reference genes should be selected. In this study, we evaluated the expressions of 11 widely used reference genes: ACTB, ATP5F1, B2M, GAPDH, HPRT1, PGK1, PPIA, RN18S1, RPLP0, TBP and UBC in 12 tissues and five brain areas of healthy common marmosets. NormFinder and geNorm indicated that the most suitable reference genes for cross-sectional studies of the 17 tissues were RN18S1 and RPLP0. Conversely, ACTB and PPIA were the most suitable for analyzing brain samples; however, the expression of PGK1 fluctuated among brain areas. These results indicate that suitable reference genes differ between the tissues examined. This study provides fundamental information for gene expression studies of the common marmoset and highlights the importance of validating reference genes before quantification of target mRNAs.

Original languageEnglish
Pages (from-to)6747-6755
Number of pages9
JournalMolecular biology reports
Volume40
Issue number12
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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