TY - JOUR
T1 - Selective Accumulation to Tumor Cells with Coacervate Droplets Formed from a Water-Insoluble Acrylate Polymer
AU - Nishida, Kei
AU - Nishimura, Shin Nosuke
AU - Tanaka, Masaru
N1 - Funding Information:
This work was financially supported by the following grants: the Research Fellowship for Young Scientists from the Japan Society for the Promotion of Science (JSPS, grant nos. JP19J00686 and 21K18066 to K.N.); Fukuoka Public Health Promotion Organization Cancer research Fund (K.N.); JSPS KAKENHI (grant no. JP19H05720 to M.T.); the Funding Program for Next-Generation World-Leading Researchers (NEXT Program) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan; and the Center of Innovation Program from the Japan Science and Technology Agency (JST). Additionally, this study was partially supported by the Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials. A part of this work was conducted in Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University, and Nanotechnology Platform Program (Molecule and Material Synthesis) of MEXT of Japan.
Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.
PY - 2022/4/11
Y1 - 2022/4/11
N2 - Selective targeting of specific cells without the use of biological ligands has not been achieved. In the present study, we revealed that the coacervate droplets formed from poly(2-methoxyethyl acrylate) (PMEA) and its derivatives selectively accumulated to tumor cells. PMEA derivatives, which are insoluble acrylate polymers, induced coacervation in water to form polymer-dense droplets via hydrophobic interaction. Interestingly, the accumulation of coacervate droplets to tumor cells was involved in the bound water content of PMEA derivatives. Coacervate droplets with a high bound water content accumulated and internalized up to 36.6-fold higher in HeLa cervical tumor cells than in normal human fibroblasts (NHDF). Moreover, the interactions between coacervate droplets and plasma membrane components such as CD44 played a key role in this accumulation process. Therefore, coacervate droplets formed from PMEA derivatives have great clinical potential in tumor cell detection, development of alternative tumor-targeting ligands, and optimization of drug delivery carriers.
AB - Selective targeting of specific cells without the use of biological ligands has not been achieved. In the present study, we revealed that the coacervate droplets formed from poly(2-methoxyethyl acrylate) (PMEA) and its derivatives selectively accumulated to tumor cells. PMEA derivatives, which are insoluble acrylate polymers, induced coacervation in water to form polymer-dense droplets via hydrophobic interaction. Interestingly, the accumulation of coacervate droplets to tumor cells was involved in the bound water content of PMEA derivatives. Coacervate droplets with a high bound water content accumulated and internalized up to 36.6-fold higher in HeLa cervical tumor cells than in normal human fibroblasts (NHDF). Moreover, the interactions between coacervate droplets and plasma membrane components such as CD44 played a key role in this accumulation process. Therefore, coacervate droplets formed from PMEA derivatives have great clinical potential in tumor cell detection, development of alternative tumor-targeting ligands, and optimization of drug delivery carriers.
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U2 - 10.1021/acs.biomac.1c01343
DO - 10.1021/acs.biomac.1c01343
M3 - Article
C2 - 35089709
AN - SCOPUS:85123998635
SN - 1525-7797
VL - 23
SP - 1569
EP - 1580
JO - Biomacromolecules
JF - Biomacromolecules
IS - 4
ER -