Selective and competitive antagonism by suramin of ATP-stimulated catecholamine-secretion from PC12 phaeochromocytoma cells

Kazuhide Inoue, K. Nakazawa, M. Ohara-Imaizumi, T. Obama, K. Fujimori, A. Takanaka

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

1. Suramin, a putative P2-antagonist, (10 to 300 μM) inhibited the adenosine 5'-triphosphate (ATP)-stimulated secretion of [3H]-noradrenaline or endogenous dopamine from phaeochromocytoma PC12 cells in a concentration-dependent manner. Suramin (300 μM) did not affect the dopamine-secretion stimulated by high K+ or nicotine. 2. Suramin shifted the concentration-response curve for ATP to the right. The antagonism was competitive with a pA2 value of 4.52. 3. ATP also stimulated an increase in intracellular Ca2+ concentration as determined by fura-2 methods. Suramin antagonized this effect over the same concentration range that antagonized the ATP-stimulated catecholamine secretion. 4. These results suggest that suramin can be used as a selective and competitive antagonist of ATP in experiments concerning mechanisms of catecholamine-secretion.

Original languageEnglish
Pages (from-to)581-584
Number of pages4
JournalBritish Journal of Pharmacology
Volume102
Issue number3
DOIs
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Suramin
PC12 Cells
Pheochromocytoma
Catecholamines
Adenosine Triphosphate
Dopamine
Fura-2
Nicotine
Norepinephrine

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Selective and competitive antagonism by suramin of ATP-stimulated catecholamine-secretion from PC12 phaeochromocytoma cells. / Inoue, Kazuhide; Nakazawa, K.; Ohara-Imaizumi, M.; Obama, T.; Fujimori, K.; Takanaka, A.

In: British Journal of Pharmacology, Vol. 102, No. 3, 1991, p. 581-584.

Research output: Contribution to journalArticle

Inoue, Kazuhide ; Nakazawa, K. ; Ohara-Imaizumi, M. ; Obama, T. ; Fujimori, K. ; Takanaka, A. / Selective and competitive antagonism by suramin of ATP-stimulated catecholamine-secretion from PC12 phaeochromocytoma cells. In: British Journal of Pharmacology. 1991 ; Vol. 102, No. 3. pp. 581-584.
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AU - Takanaka, A.

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