Abstract
1. Suramin, a putative P2-antagonist, (10 to 300 μM) inhibited the adenosine 5'-triphosphate (ATP)-stimulated secretion of [3H]-noradrenaline or endogenous dopamine from phaeochromocytoma PC12 cells in a concentration-dependent manner. Suramin (300 μM) did not affect the dopamine-secretion stimulated by high K+ or nicotine. 2. Suramin shifted the concentration-response curve for ATP to the right. The antagonism was competitive with a pA2 value of 4.52. 3. ATP also stimulated an increase in intracellular Ca2+ concentration as determined by fura-2 methods. Suramin antagonized this effect over the same concentration range that antagonized the ATP-stimulated catecholamine secretion. 4. These results suggest that suramin can be used as a selective and competitive antagonist of ATP in experiments concerning mechanisms of catecholamine-secretion.
| Original language | English |
|---|---|
| Pages (from-to) | 581-584 |
| Number of pages | 4 |
| Journal | British Journal of Pharmacology |
| Volume | 102 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1991 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Pharmacology
Cite this
Selective and competitive antagonism by suramin of ATP-stimulated catecholamine-secretion from PC12 phaeochromocytoma cells. / Inoue, Kazuhide; Nakazawa, K.; Ohara-Imaizumi, M.; Obama, T.; Fujimori, K.; Takanaka, A.
In: British Journal of Pharmacology, Vol. 102, No. 3, 1991, p. 581-584.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selective and competitive antagonism by suramin of ATP-stimulated catecholamine-secretion from PC12 phaeochromocytoma cells
AU - Inoue, Kazuhide
AU - Nakazawa, K.
AU - Ohara-Imaizumi, M.
AU - Obama, T.
AU - Fujimori, K.
AU - Takanaka, A.
PY - 1991
Y1 - 1991
N2 - 1. Suramin, a putative P2-antagonist, (10 to 300 μM) inhibited the adenosine 5'-triphosphate (ATP)-stimulated secretion of [3H]-noradrenaline or endogenous dopamine from phaeochromocytoma PC12 cells in a concentration-dependent manner. Suramin (300 μM) did not affect the dopamine-secretion stimulated by high K+ or nicotine. 2. Suramin shifted the concentration-response curve for ATP to the right. The antagonism was competitive with a pA2 value of 4.52. 3. ATP also stimulated an increase in intracellular Ca2+ concentration as determined by fura-2 methods. Suramin antagonized this effect over the same concentration range that antagonized the ATP-stimulated catecholamine secretion. 4. These results suggest that suramin can be used as a selective and competitive antagonist of ATP in experiments concerning mechanisms of catecholamine-secretion.
AB - 1. Suramin, a putative P2-antagonist, (10 to 300 μM) inhibited the adenosine 5'-triphosphate (ATP)-stimulated secretion of [3H]-noradrenaline or endogenous dopamine from phaeochromocytoma PC12 cells in a concentration-dependent manner. Suramin (300 μM) did not affect the dopamine-secretion stimulated by high K+ or nicotine. 2. Suramin shifted the concentration-response curve for ATP to the right. The antagonism was competitive with a pA2 value of 4.52. 3. ATP also stimulated an increase in intracellular Ca2+ concentration as determined by fura-2 methods. Suramin antagonized this effect over the same concentration range that antagonized the ATP-stimulated catecholamine secretion. 4. These results suggest that suramin can be used as a selective and competitive antagonist of ATP in experiments concerning mechanisms of catecholamine-secretion.
UR - http://www.scopus.com/inward/record.url?scp=0026071414&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026071414&partnerID=8YFLogxK
U2 - 10.1111/j.1476-5381.1991.tb12216.x
DO - 10.1111/j.1476-5381.1991.tb12216.x
M3 - Article
C2 - 1364823
AN - SCOPUS:0026071414
VL - 102
SP - 581
EP - 584
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
SN - 0007-1188
IS - 3
ER -