Selective autophagic receptor p62 regulates the abundance of transcriptional coregulator ARIP4 during nutrient starvation

Megumi Tsuchiya, Shin Isogai, Hiroaki Taniguchi, Hidehito Tochio, Masahiro Shirakawa, Ken Ichirou Morohashi, Yasushi Hiraoka, Tokuko Haraguchi, Hidesato Ogawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transcriptional coregulators contribute to several processes involving nuclear receptor transcriptional regulation. The transcriptional coregulator androgen receptor-interacting protein 4 (ARIP4) interacts with nuclear receptors and regulates their transcriptional activity. In this study, we identified p62 as a major interacting protein partner for ARIP4 in the nucleus. Nuclear magnetic resonance analysis demonstrated that ARIP4 interacts directly with the ubiquitin-Associated (UBA) domain of p62. ARIP4 and ubiquitin both bind to similar amino acid residues within UBA domains; therefore, these proteins may possess a similar surface structure at their UBA-binding interfaces. We also found that p62 is required for the regulation of ARIP4 protein levels under nutrient starvation conditions. We propose that p62 is a novel binding partner for ARIP4, and that its binding regulates the cellular protein level of ARIP4 under conditions of metabolic stress.

Original languageEnglish
Article number14498
JournalScientific reports
Volume5
DOIs
Publication statusPublished - Sep 28 2015

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Receptor-Interacting Protein Serine-Threonine Kinases
Androgen Receptors
Starvation
Food
Ubiquitin
Cytoplasmic and Nuclear Receptors
Proteins
Physiological Stress
Magnetic Resonance Spectroscopy
Amino Acids

All Science Journal Classification (ASJC) codes

  • General

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Selective autophagic receptor p62 regulates the abundance of transcriptional coregulator ARIP4 during nutrient starvation. / Tsuchiya, Megumi; Isogai, Shin; Taniguchi, Hiroaki; Tochio, Hidehito; Shirakawa, Masahiro; Morohashi, Ken Ichirou; Hiraoka, Yasushi; Haraguchi, Tokuko; Ogawa, Hidesato.

In: Scientific reports, Vol. 5, 14498, 28.09.2015.

Research output: Contribution to journalArticle

Tsuchiya, M, Isogai, S, Taniguchi, H, Tochio, H, Shirakawa, M, Morohashi, KI, Hiraoka, Y, Haraguchi, T & Ogawa, H 2015, 'Selective autophagic receptor p62 regulates the abundance of transcriptional coregulator ARIP4 during nutrient starvation', Scientific reports, vol. 5, 14498. https://doi.org/10.1038/srep14498
Tsuchiya, Megumi ; Isogai, Shin ; Taniguchi, Hiroaki ; Tochio, Hidehito ; Shirakawa, Masahiro ; Morohashi, Ken Ichirou ; Hiraoka, Yasushi ; Haraguchi, Tokuko ; Ogawa, Hidesato. / Selective autophagic receptor p62 regulates the abundance of transcriptional coregulator ARIP4 during nutrient starvation. In: Scientific reports. 2015 ; Vol. 5.
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