Selective detection of bacteria by a dielectrophoretic impedance measurement method using an antibody-immobilized electrode chip

Junya Suehiro, Akio Ohtsubo, Tetsuji Hatano, Masanori Hara

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

This paper describes a selective detection method for specific bacteria by using a dielectrophoretic impedance measurement method in conjunction with an antigen-antibody reaction. The authors have previously demonstrated a bacteria detection technique called DEPIM (dielectrophoretic impedance measurement) and EPA-DEPIM (electropermeabilization-assisted DEPIM) using a positive dielectrophoretic force to capture bacteria in suspension onto an interdigitated electrode chip. It has also been demonstrated that a selective DEPIM detection of specific bacteria could be realized by taking advantage of agglutination phenomena, which were caused by an antigen-antibody reaction. In the present study, other combinations of the antigen-antibody reaction with DEPIM or EPA-DEPIM were demonstrated. Antibody molecules were immobilized on the electrode chip before the preliminary bacteria trapping by positive dielectrophoresis (DEP). The bacteria were attracted to the electrode gap under the action of the positive DEP force and finally brought into contact with the glass surface to be bound with the immobilized antibody. By appropriately adjusting the balance between the DEP force and the drag force caused by liquid flow, the target bacteria (Escherichia coli) could be selectively retained on the chip surface avoiding undesired non-specific binding. The retained bacteria were electrically detected by EPA-DEPIM. It was also confirmed that the proposed method realized selective detection of the target bacteria from a mixed suspension with nontarget bacteria.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume119
Issue number1
DOIs
Publication statusPublished - Nov 24 2006

Fingerprint

Immobilized Antibodies
impedance measurement
antibodies
Antibodies
bacteria
Bacteria
chips
Electrodes
electrodes
Antigen-antibody reactions
antigens
Electrophoresis
Suspensions
agglutination
liquid flow
Escherichia
Escherichia coli
drag
Drag
adjusting

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Selective detection of bacteria by a dielectrophoretic impedance measurement method using an antibody-immobilized electrode chip. / Suehiro, Junya; Ohtsubo, Akio; Hatano, Tetsuji; Hara, Masanori.

In: Sensors and Actuators, B: Chemical, Vol. 119, No. 1, 24.11.2006, p. 319-326.

Research output: Contribution to journalArticle

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