Abstract
We proposed a new DNA detection method based on microbeads dielectrophoresis (DEP). In the method, the DNA-labeled microbeads are electrically detected. Only the DNA-labeled microbeads are trapped on a microelectrode by positive DEP, whereas the pristine ones are repelled In this paper, size selective detection of DNA using the method is described. It was found that the frequency response of DEP of the DNA-labeled microbeads depended on the DNA length. At high frequency only the long DNA-labeled microbeads were trapped, whereas the short DNA-labeled microbeads were repelled. This phenomena can realize selective detection of DNA with different length.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 2101-2103 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| Publication status | Published - Jan 1 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country | United States |
| City | San Antonio |
| Period | 10/26/14 → 10/30/14 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
Selective detection of DNA with different length using microbeads-based dielectrophoresis and impedance measurement. / Nakano, Michihiko; Kasahara, H.; Ding, Z.; Suehiro, Junya.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 2101-2103 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Selective detection of DNA with different length using microbeads-based dielectrophoresis and impedance measurement
AU - Nakano, Michihiko
AU - Kasahara, H.
AU - Ding, Z.
AU - Suehiro, Junya
PY - 2014/1/1
Y1 - 2014/1/1
N2 - We proposed a new DNA detection method based on microbeads dielectrophoresis (DEP). In the method, the DNA-labeled microbeads are electrically detected. Only the DNA-labeled microbeads are trapped on a microelectrode by positive DEP, whereas the pristine ones are repelled In this paper, size selective detection of DNA using the method is described. It was found that the frequency response of DEP of the DNA-labeled microbeads depended on the DNA length. At high frequency only the long DNA-labeled microbeads were trapped, whereas the short DNA-labeled microbeads were repelled. This phenomena can realize selective detection of DNA with different length.
AB - We proposed a new DNA detection method based on microbeads dielectrophoresis (DEP). In the method, the DNA-labeled microbeads are electrically detected. Only the DNA-labeled microbeads are trapped on a microelectrode by positive DEP, whereas the pristine ones are repelled In this paper, size selective detection of DNA using the method is described. It was found that the frequency response of DEP of the DNA-labeled microbeads depended on the DNA length. At high frequency only the long DNA-labeled microbeads were trapped, whereas the short DNA-labeled microbeads were repelled. This phenomena can realize selective detection of DNA with different length.
UR - http://www.scopus.com/inward/record.url?scp=84941687159&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941687159&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 2101
EP - 2103
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
ER -