Selective detection of DNA with different length using microbeads-based dielectrophoresis and impedance measurement

M. Nakano, H. Kasahara, Z. Ding, J. Suehiro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We proposed a new DNA detection method based on microbeads dielectrophoresis (DEP). In the method, the DNA-labeled microbeads are electrically detected. Only the DNA-labeled microbeads are trapped on a microelectrode by positive DEP, whereas the pristine ones are repelled In this paper, size selective detection of DNA using the method is described. It was found that the frequency response of DEP of the DNA-labeled microbeads depended on the DNA length. At high frequency only the long DNA-labeled microbeads were trapped, whereas the short DNA-labeled microbeads were repelled. This phenomena can realize selective detection of DNA with different length.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages2101-2103
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Fingerprint

Electrophoresis
DNA
Microelectrodes
Frequency response

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Nakano, M., Kasahara, H., Ding, Z., & Suehiro, J. (2014). Selective detection of DNA with different length using microbeads-based dielectrophoresis and impedance measurement. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2101-2103). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Selective detection of DNA with different length using microbeads-based dielectrophoresis and impedance measurement. / Nakano, M.; Kasahara, H.; Ding, Z.; Suehiro, J.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 2101-2103 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nakano, M, Kasahara, H, Ding, Z & Suehiro, J 2014, Selective detection of DNA with different length using microbeads-based dielectrophoresis and impedance measurement. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 2101-2103, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.
Nakano M, Kasahara H, Ding Z, Suehiro J. Selective detection of DNA with different length using microbeads-based dielectrophoresis and impedance measurement. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 2101-2103. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
Nakano, M. ; Kasahara, H. ; Ding, Z. ; Suehiro, J. / Selective detection of DNA with different length using microbeads-based dielectrophoresis and impedance measurement. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 2101-2103 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
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