Selective detection of NADPH among four pyridine-nucleotide cofactors by a fluorescent probe based on aggregation-induced emission

Takao Noguchi, Arnab Dawn, Daisuke Yoshihara, Youichi Tsuchiya, Tatsuhiro Yamamoto, Seiji Shinkai

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

A fluorescent sensor based on guanidinium-tethered tetraphenylethene (TPE) has been investigated toward the differentiation of pyridine nucleotide cofactors (NAD+, NADH, NADP+, and NADPH). TPE selectively recognizes NADPH possessing the higher tetra-anionic net-charge, resulting in the steep "turn-on" fluorescence increase. The comparative aggregation behaviors and fluorescence response studies of TPE on the four cofactors reveal that the critical aggregate concentration of TPE against NADPH correlates directly with the concentration threshold for the fluorescence response. These results establish that TPE can selectively differentiate NADPH over the other three cofactors by the steep aggregation-induced fluorescence response accompanied by the high signal-to-background contrast.

Original languageEnglish
Pages (from-to)779-784
Number of pages6
JournalMacromolecular rapid communications
Volume34
Issue number9
DOIs
Publication statusPublished - May 14 2013

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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