Selective determination of amitriptyline and nortriptyline in human plasma by HPLC with ultraviolet and particle beam mass spectrometry

Keiko Kudo, Narumi Jitsufuchi, Tohru Imamura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A selective, sensitive, and reliable method was devised to determine concentrations of amitriptyline and its major metabolite, nortriptyline, in human plasma using high-performance liquid chromatography (HPLC) combined with UV and particle beam mass spectrometry (PBMS). Amitriptyline and nortriptyline were effectively extracted in a three-step solvent extraction procedure. Imipramine was used as the internal standard (IS). Amitriptyline, nortriptyline, and the IS were clearly separated by HPLC on a silica column with the mobile phase of acetonitrile-0.1M ammonium acetate (94:6, v/v). The calibration curves were linear in the concentration range of 10-1000 ng/g for both compounds with UV and PBMS detections. The lower limits of detection were 5 ng/g for amitriptyline and 10 ng/g for nortriptyline with UV detection and 2 ng/g for amitriptyline and 5 ng/g for nortriptyline with PBMS detection. The absolute recoveries were 58% for amitriptyline and 47% for nortriptyline at a concentration of 50 ng/g. This method proved most useful in accurately identifying amitriptyline and nortriptyline in tissues from an autopsied individual.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalJournal of Analytical Toxicology
Volume21
Issue number3
DOIs
Publication statusPublished - Jan 1 1997

Fingerprint

Nortriptyline
Plasma (human)
Particle beams
Amitriptyline
High performance liquid chromatography
Mass spectrometry
liquid chromatography
Mass Spectrometry
mass spectrometry
High Pressure Liquid Chromatography
plasma
Solvent extraction
Metabolites
Acetonitrile
Silica
Calibration
Tissue
Recovery
metabolite
acetate

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Cite this

Selective determination of amitriptyline and nortriptyline in human plasma by HPLC with ultraviolet and particle beam mass spectrometry. / Kudo, Keiko; Jitsufuchi, Narumi; Imamura, Tohru.

In: Journal of Analytical Toxicology, Vol. 21, No. 3, 01.01.1997, p. 185-189.

Research output: Contribution to journalArticle

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