Selective extraction and recovery of cytochrome c by liquid-liquid extraction using a calix[6]arene carboxylic acid derivative

Tatsuya Oshima, Hiroaki Higuchi, Keisuke Ohto, Katsutoshi Inoue, Masahiro Goto

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Recently, we reported that a calix[6]arene carboxylic acid derivative can selectively extract the lysinerich protein cytochrome c by interacting with amino groups on the protein surface. In the present article, quantitative extraction and recovery of cytochrome c using this calix[6]arene carboxylic acid derivative are described. Both adjustment of the pH under acidic conditions and addition of an alcohol are necessary to strip the extracted protein from an organic solution to an aqueous solution. Separation of cytochrome c and lysozyme using the calix[6] arene was achieved under the optimal conditions. In the forward extraction stage, 93% of the cytochrome c was extracted, while lysozyme remained in the solution. In the subsequent stripping stage, the extracted cytochrome c was quantitatively recovered in an aqueous solution. Finally, separation of these proteins, which have similar molecular weights and isoelectric points, was accomplished.

Original languageEnglish
Pages (from-to)7280-7284
Number of pages5
JournalLangmuir
Volume21
Issue number16
DOIs
Publication statusPublished - Aug 2 2005

Fingerprint

cytochromes
Carboxylic Acids
Cytochromes c
Carboxylic acids
carboxylic acids
recovery
Derivatives
Proteins
Recovery
Liquids
proteins
liquids
lysozyme
Muramidase
aqueous solutions
stripping
Enzymes
molecular weight
strip
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Selective extraction and recovery of cytochrome c by liquid-liquid extraction using a calix[6]arene carboxylic acid derivative. / Oshima, Tatsuya; Higuchi, Hiroaki; Ohto, Keisuke; Inoue, Katsutoshi; Goto, Masahiro.

In: Langmuir, Vol. 21, No. 16, 02.08.2005, p. 7280-7284.

Research output: Contribution to journalArticle

Oshima, Tatsuya ; Higuchi, Hiroaki ; Ohto, Keisuke ; Inoue, Katsutoshi ; Goto, Masahiro. / Selective extraction and recovery of cytochrome c by liquid-liquid extraction using a calix[6]arene carboxylic acid derivative. In: Langmuir. 2005 ; Vol. 21, No. 16. pp. 7280-7284.
@article{295a008798ac441bb408df401bc69614,
title = "Selective extraction and recovery of cytochrome c by liquid-liquid extraction using a calix[6]arene carboxylic acid derivative",
abstract = "Recently, we reported that a calix[6]arene carboxylic acid derivative can selectively extract the lysinerich protein cytochrome c by interacting with amino groups on the protein surface. In the present article, quantitative extraction and recovery of cytochrome c using this calix[6]arene carboxylic acid derivative are described. Both adjustment of the pH under acidic conditions and addition of an alcohol are necessary to strip the extracted protein from an organic solution to an aqueous solution. Separation of cytochrome c and lysozyme using the calix[6] arene was achieved under the optimal conditions. In the forward extraction stage, 93{\%} of the cytochrome c was extracted, while lysozyme remained in the solution. In the subsequent stripping stage, the extracted cytochrome c was quantitatively recovered in an aqueous solution. Finally, separation of these proteins, which have similar molecular weights and isoelectric points, was accomplished.",
author = "Tatsuya Oshima and Hiroaki Higuchi and Keisuke Ohto and Katsutoshi Inoue and Masahiro Goto",
year = "2005",
month = "8",
day = "2",
doi = "10.1021/la050364a",
language = "English",
volume = "21",
pages = "7280--7284",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "16",

}

TY - JOUR

T1 - Selective extraction and recovery of cytochrome c by liquid-liquid extraction using a calix[6]arene carboxylic acid derivative

AU - Oshima, Tatsuya

AU - Higuchi, Hiroaki

AU - Ohto, Keisuke

AU - Inoue, Katsutoshi

AU - Goto, Masahiro

PY - 2005/8/2

Y1 - 2005/8/2

N2 - Recently, we reported that a calix[6]arene carboxylic acid derivative can selectively extract the lysinerich protein cytochrome c by interacting with amino groups on the protein surface. In the present article, quantitative extraction and recovery of cytochrome c using this calix[6]arene carboxylic acid derivative are described. Both adjustment of the pH under acidic conditions and addition of an alcohol are necessary to strip the extracted protein from an organic solution to an aqueous solution. Separation of cytochrome c and lysozyme using the calix[6] arene was achieved under the optimal conditions. In the forward extraction stage, 93% of the cytochrome c was extracted, while lysozyme remained in the solution. In the subsequent stripping stage, the extracted cytochrome c was quantitatively recovered in an aqueous solution. Finally, separation of these proteins, which have similar molecular weights and isoelectric points, was accomplished.

AB - Recently, we reported that a calix[6]arene carboxylic acid derivative can selectively extract the lysinerich protein cytochrome c by interacting with amino groups on the protein surface. In the present article, quantitative extraction and recovery of cytochrome c using this calix[6]arene carboxylic acid derivative are described. Both adjustment of the pH under acidic conditions and addition of an alcohol are necessary to strip the extracted protein from an organic solution to an aqueous solution. Separation of cytochrome c and lysozyme using the calix[6] arene was achieved under the optimal conditions. In the forward extraction stage, 93% of the cytochrome c was extracted, while lysozyme remained in the solution. In the subsequent stripping stage, the extracted cytochrome c was quantitatively recovered in an aqueous solution. Finally, separation of these proteins, which have similar molecular weights and isoelectric points, was accomplished.

UR - http://www.scopus.com/inward/record.url?scp=23844477981&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23844477981&partnerID=8YFLogxK

U2 - 10.1021/la050364a

DO - 10.1021/la050364a

M3 - Article

C2 - 16042454

AN - SCOPUS:23844477981

VL - 21

SP - 7280

EP - 7284

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 16

ER -