Selective fluorescence detection of histamine based on ligand exchange mechanism and its application to biomonitoring

Daisuke Seto, Nobuaki Soh, Koji Nakano, Toshihiko Imato

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni2+ and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni2+ ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity toward histamine among the various neurotransmitters in 0.1M phosphate buffer solution (pH 7.4). Biomonitoring studies to detect histamine released from RAW264 cells are successfully represented.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalAnalytical Biochemistry
Volume404
Issue number2
DOIs
Publication statusPublished - Sep 2010

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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