Selective fluorescence detection of histamine based on ligand exchange mechanism and its application to biomonitoring

Daisuke Seto, Nobuaki Soh, Koji Nakano, Toshihiko Imato

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni2+ and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni2+ ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity toward histamine among the various neurotransmitters in 0.1M phosphate buffer solution (pH 7.4). Biomonitoring studies to detect histamine released from RAW264 cells are successfully represented.

    Original languageEnglish
    Pages (from-to)135-139
    Number of pages5
    JournalAnalytical Biochemistry
    Volume404
    Issue number2
    DOIs
    Publication statusPublished - Sep 2010

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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