Selective fluorescence quenching of the 8-oxoG-clamp by 8-oxodeoxyguanosine in ODN

Tamer Nasr, Zhichun Li, Osamu Nakagawa, Yosuke Taniguchi, Sayaka Ono, Shigeki Sasaki

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The 8-oxoG-clamp, a specific fluorescent probe for 8-oxo-deoxyguanosine (8-oxo-dG), was incorporated into the oligodeoxynucleotide (ODN) within or at the 3′-end of the purine and the pyrimidine sequences. Based on the UV-melting temperature, the 8-oxoG-clamp showed slightly lower stabilizing effects on the duplexes containing 8-oxo-dG at the complementary site than that with dG. On the other hand, 8-oxo-dG in DNA was selectively detected by fluorescence quenching of the 8-oxoG-clamp.

Original languageEnglish
Pages (from-to)727-730
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number3
DOIs
Publication statusPublished - Feb 1 2009

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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